基于四面体 DNA 纳米结构的 FRET 效应编码荧光强度用于生物检测。
Encoding fluorescence intensity with tetrahedron DNA nanostructure based FRET effect for bio-detection.
机构信息
National Key Laboratory of Materials for Integrated Circuits, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai, 200050, China; School of Physics and Optoelectronic Engineering, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China; University of Chinese Academy of Science, Beijing, 100049, China.
National Key Laboratory of Materials for Integrated Circuits, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai, 200050, China; Shanghai Advanced Research Institute, Chinese Academy of Science, Shanghai, 201210, China.
出版信息
Biosens Bioelectron. 2024 Mar 15;248:115994. doi: 10.1016/j.bios.2023.115994. Epub 2023 Dec 30.
Biocoding technology constructed by readable tags with distinct signatures is a brand-new bioanalysis method to realize multiplexed identification and bio-information decoding. In this study, a novel fluorescence intensity coding technology termed Tetra-FICT was reported based on tetrahedron DNA nanostructure (TDN) carrier and Főrster Resonance Energy Transfer (FRET) effect. By modulating numbers and distances of Cy3 and Cy5 at four vertexes of TDN, different fluorescence intensities of twenty-six samples were produced at ∼565.0 nm (FI) and ∼665.0 nm (FI) by detecting fluorescence spectra. By developing an error correction mechanism, eleven codes were established based on divided intensity ranges of the final FI together with FI (Final-FI&FI). These resulting codes were used to construct barcode probes, with three miRNA biomarkers (miRNA-210, miRNA-199a and miRNA-21) as cases for multiplexed bio-assay. The high specificity and sensitivity were also demonstrated for the detection of miRNA-210. Overall, the proposed Tetra-FICT enriched the toolbox of fluorescence coding, which could be applied to multiplexing biomarkers detection.
生物编码技术通过具有独特特征的可读标签构建，是一种实现多重识别和生物信息解码的全新生物分析方法。本研究基于四面体 DNA 纳米结构（TDN）载体和Förster 共振能量转移（FRET）效应，报道了一种新型荧光强度编码技术，称为 Tetra-FICT。通过调节 TDN 四个顶点处 Cy3 和 Cy5 的数量和距离，在 ∼565.0nm（FI）和 ∼665.0nm（FI）处通过检测荧光光谱产生了 26 个样品的不同荧光强度（FI）。通过开发纠错机制，基于最终 FI 和 FI（Final-FI&FI）的强度范围划分建立了 11 个代码。这些生成的代码被用于构建条码探针，以三种 miRNA 生物标志物（miRNA-210、miRNA-199a 和 miRNA-21）为例进行多重生物测定。还证明了检测 miRNA-210 的高特异性和灵敏度。总之，所提出的 Tetra-FICT 丰富了荧光编码工具包，可应用于多重生物标志物检测。
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