Geißler Daniel, Hildebrandt Niko
Division 1.10 Biophotonics, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489, Berlin, Germany.
NanoBioPhotonics (www.nanofret.com), Institut d'Electronique Fondamentale, Université Paris-Sud, CNRS, Université Paris-Saclay, 91405, Orsay cedex, France.
Anal Bioanal Chem. 2016 Jul;408(17):4475-83. doi: 10.1007/s00216-016-9434-y. Epub 2016 Mar 12.
The exceptional photophysical properties and the nanometric dimensions of colloidal semiconductor quantum dots (QD) have strongly attracted the bioanalytical community over the last approximately 20 y. In particular, the integration of QDs in the analysis of biological components and interactions, and the related diagnostics using Förster resonance energy transfer (FRET), have allowed researchers to significantly improve and diversify fluorescence-based biosensing. In this TRENDS article, we review some recent developments in QD-FRET biosensing that have implemented this technology in electronic consumer products, multiplexed analysis, and detection without light excitation for diagnostic applications. In selected examples of smartphone-based imaging, single- and multistep FRET, steady-state and time-resolved spectroscopy, and bio/chemiluminescence detection of QDs used as both FRET donors and acceptors, we highlight the advantages of QD-based FRET biosensing for multiplexed and sensitive diagnostics. Graphical Abstract Quantum dots (QDs) can be applied as donors and/or acceptors for Förster resonance energy transfer- (FRET-) based biosensing for multiplexed and sensitive diagnostics in various assay formats.
在过去约20年里，胶体半导体量子点（QD）卓越的光物理性质和纳米尺寸强烈吸引了生物分析领域。特别是，量子点在生物成分和相互作用分析中的应用，以及使用福斯特共振能量转移（FRET）的相关诊断方法，使研究人员能够显著改进基于荧光的生物传感并使其多样化。在这篇《趋势》文章中，我们回顾了量子点-福斯特共振能量转移生物传感的一些最新进展，这些进展已将该技术应用于电子产品、多重分析以及无需光激发的诊断应用检测中。在基于智能手机成像、单步和多步福斯特共振能量转移、稳态和时间分辨光谱以及用作福斯特共振能量转移供体和受体的量子点的生物/化学发光检测的选定示例中，我们强调了基于量子点的福斯特共振能量转移生物传感在多重和灵敏诊断方面的优势。图形摘要 量子点可作为供体和/或受体用于基于福斯特共振能量转移（FRET）的生物传感，以在各种检测形式中进行多重和灵敏诊断。
