Hoke R A, Deerfield D W, Pedersen L G, Koehler K A, Hiskey R G
Int J Pept Protein Res. 1986 Dec;28(6):569-78. doi: 10.1111/j.1399-3011.1986.tb03294.x.
The vitamin K-dependent proteins involved in blood coagulation processes each contain a small cystine loop with the sequence -Gla-Cys-X-Gla-Gla-X-Cys-. A method for the synthesis of the heptapeptide derivative representing the 17-23 sequence of bovine prothrombin, Z-Gla-Cys-Leu-Gla-Gla-Pro-Cys-OBzl, has been developed. The 25Mg2+ n.m.r. spectrum of the heptapeptide derivative has been obtained and is compared to the n.m.r. spectra obtained from the interaction of 25Mg2+ with Z-Gla-OMe and Z-Gla-Gla-OMe.
参与血液凝固过程的维生素K依赖蛋白均含有一个小的胱氨酸环，其序列为-Gla-Cys-X-Gla-Gla-X-Cys-。已开发出一种合成代表牛凝血酶原17 - 23序列的七肽衍生物Z-Gla-Cys-Leu-Gla-Gla-Pro-Cys-OBzl的方法。已获得该七肽衍生物的25Mg2+核磁共振谱，并将其与25Mg2+与Z-Gla-OMe和Z-Gla-Gla-OMe相互作用所获得的核磁共振谱进行比较。
