Lab in Microbiology and Marine Biotechnology, Department of Biotechnology, School of Biological Sciences, Alagappa University, Karaikudi 630 003, India.
Lab in Microbiology and Marine Biotechnology, Department of Biotechnology, School of Biological Sciences, Alagappa University, Karaikudi 630 003, India; The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
Int J Biol Macromol. 2024 Feb;259(Pt 1):129161. doi: 10.1016/j.ijbiomac.2023.129161. Epub 2024 Jan 4.
Antibacterial hydrogels have emerged as a promising approach for wound healing, owing to their ability to integrate antibacterial agents into the hydrogel matrix. Benefiting from its remarkable antibacterial and wound-healing attributes, pyrogallol has been introduced into chitosan-gelatin for the inaugural development of an innovative antibacterial polymeric hydrogel tailored for applications in wound healing. Hence, we observed the effectiveness of pyrogallol in inhibiting the growth of A. baumannii, disrupting mature biofilms, and showcasing robust antioxidant activity both in vitro and in vivo. In addition, pyrogallol promoted the migration of human epidermal keratinocytes and exhibited wound healing activity in zebrafish. These findings suggest that pyrogallol holds promise as a therapeutic agent for wound healing. Interestingly, the pyrogallol-loaded chitosan-gelatin (Pyro-CG) hydrogel exhibited enhanced mechanical strength, stability, controlled drug release, biodegradability, antibacterial activity, and biocompatibility. In vivo results established that Pyro-CG hydrogel promotes wound closure and re-epithelialization in A. baumannii-induced wounds in molly fish. Therefore, the prepared Pyro-CG polymeric hydrogel stands poised as a potent and promising agent for wound healing with antibacterial properties. This holds considerable promise for the development of effective therapeutic interventions to address the increasing menace of A. baumannii-induced wound infections.
抗菌水凝胶作为一种有前途的伤口愈合方法，由于其能够将抗菌剂整合到水凝胶基质中而备受关注。焦性没食子酸由于其显著的抗菌和伤口愈合特性，已被引入壳聚糖-明胶中，首次开发出一种针对伤口愈合应用的创新抗菌聚合物水凝胶。因此，我们观察到焦性没食子酸抑制鲍曼不动杆菌生长、破坏成熟生物膜以及在体外和体内表现出强大抗氧化活性的效果。此外，焦性没食子酸促进人表皮角质形成细胞的迁移，并在斑马鱼中表现出伤口愈合活性。这些发现表明焦性没食子酸有望成为一种治疗伤口愈合的药物。有趣的是，负载焦性没食子酸的壳聚糖-明胶（Pyro-CG）水凝胶表现出增强的机械强度、稳定性、控释药物、生物降解性、抗菌活性和生物相容性。体内结果表明，Pyro-CG 水凝胶促进了莫利鱼中鲍曼不动杆菌诱导伤口的闭合和再上皮化。因此，制备的 Pyro-CG 聚合物水凝胶有望成为一种具有抗菌性能的有效治疗干预的有力候选药物。这为开发有效治疗方法来应对日益严重的鲍曼不动杆菌诱导的伤口感染提供了巨大的潜力。
