Robinson A S, Pham V L
Heredity (Edinb). 1987 Feb;58 ( Pt 1):95-101. doi: 10.1038/hdy.1987.14.
T(Y-D1)35 is a genetic sexing line in Anopheles stephensi (2n = 6; XX female, XY male) based on the translocation of the dieldrin resistance gene to the male determining chromosome. A cytological analysis demonstrated the presence of a 3 chromosome multiple translocation in which part of the Y chromosome formed the differential segment. An analysis of egg karyotypes enabled the segregation behaviour of the complex to be determined and only alternate and adjacent I segregations could be demonstrated. The breakpoint of the translocation in chromosome 3 was located using polytene salivary gland preparations. Linkage studies using chromosome three markers revealed contrasting effects of the translocation on recombination in this chromosome. An insecticide treatment method, based on the exposure of newly emerged adults, was developed enabling large numbers of individuals to be treated for the production of only males.
T(Y-D1)35是斯氏按蚊（2n = 6；XX为雌性，XY为雄性）中的一条遗传性别鉴定品系，它基于狄氏剂抗性基因易位到雄性决定染色体上。细胞学分析表明存在一种3染色体多重易位，其中Y染色体的一部分形成了差异区段。对卵核型的分析能够确定该复合体的分离行为，并且仅能证明交替分离和相邻I分离。利用多线唾液腺制备物定位了3号染色体上易位的断点。使用3号染色体标记的连锁研究揭示了易位对该染色体重组的不同影响。开发了一种基于刚羽化成虫暴露的杀虫剂处理方法，可处理大量个体以仅生产雄性个体。
