La Fleur M, Beaulieu A D, Kreis C, Poubelle P
J Biol Chem. 1987 Feb 15;262(5):2111-5.
Using a fibronectin cDNA probe, we have studied the accumulation of fibronectin mRNA in polymorphonuclear leukocytes (PMN) in response to inflammation. Nonactivated PMN from human peripheral blood were used as a source of noninflammatory cells and PMN from inflamed knee joints of patients with chronic inflammatory joint disorders (rheumatoid and psoriatic arthritis) were used as a source of inflammatory cells. By dot blot and Northern hybridization analysis, we have found the presence of fibronectin mRNA in these cells. Its size was estimated at approximately equal to 8.7-8.8 kilobases. When noninflammatory PMN were compared to inflammatory PMN in terms of fibronectin mRNA accumulation, a marked increase was found in inflammatory cells (2- to 12.7-fold stimulation). It was also observed that the increased mRNA levels in inflammatory PMN lead to increased synthesis of the protein. These findings establish that PMN are part of the fibronectin-producing cells and that the level of mRNA in these cells is influenced by the inflammatory process.
利用纤连蛋白cDNA探针，我们研究了多形核白细胞（PMN）中纤连蛋白mRNA在炎症反应中的积累情况。来自人类外周血的未激活PMN被用作非炎症细胞的来源，而来自慢性炎症性关节疾病（类风湿性关节炎和银屑病关节炎）患者发炎膝关节的PMN被用作炎症细胞的来源。通过斑点印迹和Northern杂交分析，我们在这些细胞中发现了纤连蛋白mRNA的存在。其大小估计约为8.7 - 8.8千碱基。当比较非炎症PMN和炎症PMN中纤连蛋白mRNA的积累时，发现炎症细胞中有明显增加（刺激2至12.7倍）。还观察到炎症PMN中增加的mRNA水平导致蛋白质合成增加。这些发现证实PMN是产生纤连蛋白的细胞的一部分，并且这些细胞中mRNA的水平受炎症过程影响。
