Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781 039, India.
J Biosci. 2024;49.
The cation diffusion facilitator (CDF) family of zinc transporters plays a crucial role in zinc homeostasis in eukaryotes, including fungi. Here, we investigated the cell functions and genetic interactions of CDF zinc transporters -1 and -2 in . The Δ-1 mutant could not grow in a high-zinc environment, indicating that the zinc transporter protein ZRC-1 was essential for growth in high-zinc conditions. However, the deletion of -2 did not show any severe phenotypic defects. Furthermore, we studied the genetic interactions of the zinc transporters using the CDF double mutants. Previously, -17 was reported to be critical, where the Δ-17 mutant showed defects in both vegetative development and asexual sporulation. Interestingly, the Δ-2;Δ-17 double mutant showed phenotypes similar to the wild type, and restored the phenotypic defects of the Δzrg-17 mutation. However, the Δ;Δ and Δ;Δ double mutants continue to display phenotypic defects like their parental single mutants. The double mutant Δzrc-1;Δzrg-17 showed severe vegetative growth defects, including slow growth, short aerial hyphae, narrowed septation, and defective asexual sporulation. In addition, aerial hyphae development of the double mutants were reduced under endoplasmic reticulum stress. Thus, this study revealed the cell functions and genetic interactions of for vegetative development and tolerance to stress conditions in
阳离子扩散促进因子（CDF）家族的锌转运蛋白在真核生物（包括真菌）的锌稳态中起着至关重要的作用。在这里，我们研究了 CDF 锌转运蛋白 -1 和 -2 在中的细胞功能和遗传相互作用。Δ-1 突变体在高锌环境中无法生长，表明锌转运蛋白 ZRC-1 对于高锌条件下的生长是必需的。然而，-2 的缺失并没有显示出任何严重的表型缺陷。此外，我们使用 CDF 双突变体研究了锌转运体的遗传相互作用。先前有报道称 -17 是关键的，Δ-17 突变体在营养生长和无性孢子形成中都表现出缺陷。有趣的是，Δ-2；Δ-17 双突变体表现出与野生型相似的表型，并且恢复了 Δzrg-17 突变的表型缺陷。然而，Δ；Δ 和 Δ；Δ 双突变体继续表现出与其亲本单突变体相似的表型缺陷。双突变体 Δzrc-1；Δzrg-17 表现出严重的营养生长缺陷，包括生长缓慢、气生菌丝短、隔膜变窄和无性孢子形成缺陷。此外，双突变体的气生菌丝发育在内质网应激下减少。因此，本研究揭示了 对于营养生长和对胁迫条件的耐受性的细胞功能和遗传相互作用
