Zou Deng, Yuan Jun, Ding Jie, Li Jun, Zhang Haijun
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Mitochondrial DNA B Resour. 2023 Nov 14;8(11):1248-1252. doi: 10.1080/23802359.2023.2278820. eCollection 2023.
Bremer & Grey, 1853 belongs to the lepidopteran family Geometridae, subfamily Ennominae. We sequenced the complete mitochondrial genome of by PCR and Sanger sequencing method. The mitochondrial genome of is 15,523 bp in length and contains a typical set of 37 genes with 'MIQ' type gene arrangement and a 394 bp AT-rich regions. Except for using CGA as initiation codon, all protein-coding genes (PCGs) start with ATN codons and except for and using TAG as termination codon, all PCGs terminated with TAA codon. A phylogenetic tree including 39 genus of subfamily Ennominae was first reconstructed based on the mitochondrial genome sequences with nucleotide substitution model GTR + G + I, which showed that the genera , , and are not monophyletic and and are classified together.
布雷默灰尺蛾(Bremer & Grey, 1853)属于鳞翅目尺蛾科枝尺蛾亚科。我们采用聚合酶链式反应(PCR)和桑格测序法对其线粒体基因组进行了全序列测定。布雷默灰尺蛾的线粒体基因组长度为15,523碱基对,包含一组典型的37个基因,其基因排列为“MIQ”型,并含有一个394碱基对的富含AT区域。除了某基因使用CGA作为起始密码子外,所有蛋白质编码基因(PCGs)均以ATN密码子起始;除了某两个基因使用TAG作为终止密码子外,所有PCGs均以TAA密码子终止。基于线粒体基因组序列,采用核苷酸替换模型GTR + G + I,首次构建了包含枝尺蛾亚科39个属的系统发育树,结果表明某几个属并非单系类群,且某两个属被归为一类。 (注:原文中部分基因名称未明确给出,翻译时用“某”指代)