Sun Y U, Zhang Jiawei, Li Qingqing, Liang Dan, Abbas Muhammad Nadeem, Qian Cen, Wang Lei, Wei Guoqing, Zhu Bao-Jian, Liu Chao-Liang
College of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Zootaxa. 2017 Apr 20;4254(5):501-519. doi: 10.11646/zootaxa.4254.5.1.
In this study, a complete mitochondrial genome (mitogenome) sequence of Abraxas suspecta (Lepidoptera: Geometridae) is isolated and characterized. The complete DNA is 15,547 bp length and contains 2 ribosomal RNA genes, 23 putative transfer RNA (tRNA) genes including an extra tRNAAsn (AUU), 13 protein-coding genes and an adenine (A) + thymine (T)-rich region. The nucleotide composition and gene organization are identical to those of other lepidopteran, except for the presence of an extra copy of trnN (AUU). Of the 38 genes, twenty-five genes (9 PCGs and 16 tRNAs) are encoded by heavy strand (H-strand), while thirteen are encoded by light strand (L-strand). Among the 13 PCGs, 12 PCGs employ ATN as initiation codon, while cytochrome c oxidase subunit 1 (cox1) utilizes CGA as initiation codon. Four of the 13 PCGs have the incomplete termination codon T, while the remainder terminated with the canonical stop codon. All tRNA genes are folded into the typical clover-leaf structure of mitochondrial tRNAs, except for the tRNASer (AGN) gene, in which the DHU arm fails to form a stable stem-loop structure. The A+T-rich region is 532 bp long, and contains some conserved regions, including 'ATAGA' motif followed by a 17bp poly-T stretch, a microsatellite-like element (AT)8(AAT)3 and also a poly-A element. A short Phylogenetic analysis based on 13 PCGs using maximum likelihood (ML) and Bayesian inference (BI) revealed that A. suspecta resides in the Geometridae family. We present the method and approach to use moths as model organisms for further genetic and evolutionary biology studies.
在本研究中，分离并鉴定了疑似桦尺蛾（鳞翅目：尺蛾科）的完整线粒体基因组（mitogenome）序列。完整的DNA长度为15,547 bp，包含2个核糖体RNA基因、23个推定的转移RNA（tRNA）基因，其中包括一个额外的tRNAAsn（AUU）、13个蛋白质编码基因和一个富含腺嘌呤（A）+胸腺嘧啶（T）的区域。除了存在额外的trnN（AUU）拷贝外，其核苷酸组成和基因组织与其他鳞翅目昆虫相同。在这38个基因中，25个基因（9个蛋白质编码基因和16个tRNA）由重链（H链）编码，而13个由轻链（L链）编码。在13个蛋白质编码基因中，12个蛋白质编码基因使用ATN作为起始密码子，而细胞色素c氧化酶亚基1（cox1）利用CGA作为起始密码子。13个蛋白质编码基因中有4个具有不完全终止密码子T，其余的则以典型的终止密码子终止。除了tRNASer（AGN）基因外，所有tRNA基因都折叠成线粒体tRNA典型的三叶草叶结构，其中DHU臂未能形成稳定的茎环结构。富含A+T的区域长532 bp，包含一些保守区域，包括“ATAGA”基序，其后是一个17 bp的多聚T序列、一个微卫星样元件（AT）8（AAT）3以及一个多聚A元件。基于13个蛋白质编码基因使用最大似然法（ML）和贝叶斯推断（BI）进行的简短系统发育分析表明，疑似桦尺蛾属于尺蛾科。我们提出了将蛾类用作进一步遗传和进化生物学研究的模式生物的方法和途径。
