Bastiras S, Calvert G D
J Chromatogr. 1986 Nov 28;383(1):27-34. doi: 10.1016/s0378-4347(00)83437-4.
A system for the isolation of human plasma lipid transfer protein (LTP) has been devised using a combination of conventional and high-performance ion-exchange chromatography. Following initial purification by ammonium sulphate precipitation, ultracentrifugation, hydrophobic interaction and cation-exchange chromatography, appropriate fractions were further purified using the Pharmacia fast protein liquid chromatography system. Using this method of purification, human plasma LTP has been purified more rapidly and with greater recovery than with conventional column chromatography. Whereas two forms of LTP were previously reported from the authors' laboratory [LTP-I, molecular mass (Mr) 69,000 and LTP-II, Mr 55,000], with an improved chromatographic system only one form of LTP (LTP-I) has been isolated. This suggests that LTP-II may have been a fragment of LTP-I, produced during the previously used lengthy purification process.
已设计出一种利用传统离子交换色谱法与高效离子交换色谱法相结合的方法来分离人血浆脂质转运蛋白（LTP）的系统。通过硫酸铵沉淀、超速离心、疏水相互作用和阳离子交换色谱法进行初步纯化后，使用Pharmacia快速蛋白质液相色谱系统对合适的馏分进行进一步纯化。采用这种纯化方法，与人血浆LTP的传统柱色谱法相比，其纯化速度更快，回收率更高。虽然作者实验室之前报道过两种形式的LTP [LTP-I，分子量（Mr）69,000和LTP-II，Mr 55,000]，但使用改进的色谱系统仅分离出一种形式的LTP（LTP-I）。这表明LTP-II可能是LTP-I的一个片段，是在之前使用的冗长纯化过程中产生的。
