Kaji Takuya, Matsumoto Kotaro, Okumura Taichi, Nakayama Misuzu, Hoshino Shunji, Takaoka Yousuke, Wang Jianxin, Ueda Minoru
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan.
Department of Molecular and Chemical Life Sciences, Graduate School of Life Science, Tohoku University, Sendai 980-8578, Japan.
iScience. 2023 Dec 3;27(1):108625. doi: 10.1016/j.isci.2023.108625. eCollection 2024 Jan 19.
The plant hormone (3, 7)-jasmonoyl-L-isoleucine ((3, 7)-JA-Ile) is perceived by the COI1-JAZ co-receptor in , leading to the activation of gene expression for plant defense responses, growth, development, and other processes. Therefore, understanding the interaction between the COI1-JAZ co-receptor and its ligands is essential for the development of COI1-JAZ agonists and antagonists as potent chemical tools for regulating (3, 7)-JA-Ile signaling. This study demonstrated that COI1-JAZ has two independent modes of ligand perception using a differential scanning fluorimetry (DSF) assay. (3, 7)-JA-Ile is perceived through a one-step model in which (3, 7)-JA-Ile causes protein-protein interaction between COI1 and JAZ. In contrast, coronatine (COR), a mimic of (3, 7)-JA-Ile, is perceived through a two-step model in which COR is first perceived by COI1 and then recruits JAZ to form the COI1-COR-JAZ complex. Our results demonstrate two distinct modes of action of molecular glues causing protein-protein interactions.
植物激素（3,7）-茉莉酰-L-异亮氨酸（（3,7）-JA-Ile）在植物中被COI1-JAZ共受体识别，从而激活植物防御反应、生长、发育及其他过程的基因表达。因此，了解COI1-JAZ共受体与其配体之间的相互作用对于开发COI1-JAZ激动剂和拮抗剂至关重要，这些激动剂和拮抗剂可作为调节（3,7）-JA-Ile信号传导的有效化学工具。本研究使用差示扫描荧光法（DSF）分析表明，COI1-JAZ具有两种独立的配体识别模式。（3,7）-JA-Ile通过一步模型被识别，其中（3,7）-JA-Ile导致COI1和JAZ之间发生蛋白质-蛋白质相互作用。相比之下，（3,7）-JA-Ile的类似物冠菌素（COR）通过两步模型被识别，其中COR首先被COI1识别，然后招募JAZ形成COI1-COR-JAZ复合物。我们的结果证明了导致蛋白质-蛋白质相互作用的分子胶水的两种不同作用模式。
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