Centro de Investigación Aplicada en Ambiente y Salud (CIAAS), Universidad Autónoma de San Luis Potosí, Avenida Sierra Leona No. 550, Colonia Lomas Segunda Sección CP 78210, San Luis Potosí, SLP, Mexico.
Instituto Tecnológico Superior de Rioverde, Carretera Rioverde-San Ciro Km 4.5, Rioverde CP. 79610, San Luis Potosi, Mexico.
Eur J Pharm Biopharm. 2024 Feb;195:114178. doi: 10.1016/j.ejpb.2024.114178. Epub 2024 Jan 7.
The aim of this study was the molecular imprinting polymers (MIPs) assessment as a controlled release system of ciprofloxacin. The MIPs synthesis was performed by three different methods: emulsion, bulk, and co-precipitation. Lactic acid (LA) and methacrylic acid (MA) were used as functional monomers and ethylene glycol dimethacrylate as crosslinker. Also, nonimprinted polymers (NIPs) were synthesized. MIPs and NIPs were characterized by scanning electron microscopy, Fourier Transform Infrared Reflection, specific surface area, pore size, and release kinetics. Their efficiency against Staphylococcus aureus and Escherichia coli, and their cytotoxicity in dermal fibroblast cells were proven. Results show that MIPs are mesoporous materials with a pore size between 10 and 20 nm. A higher adsorption with the co-precipitation MIP with MA as a monomer was found. The release kinetics proved that a non-Fickian process occurred and that the co-precipitation MIP with LA presented the highest release rate (90.51 mg/L) in 8 h. The minimum inhibitory concentration was found between 0.031 and 0.016 mg/L for Staphylococcus aureus and between 0.004 and 0.031 mg/L for the Escherichia coli. No cytotoxicity in cellular cultures was found; also, cellular growth was favored. This study demonstrated that MIPs present promising properties for drug administration and their application in clinical practice.
本研究旨在评估分子印迹聚合物（MIPs）作为环丙沙星控释系统的性能。采用三种不同方法：乳液法、本体聚合法和共沉淀法合成 MIPs。使用乳酸（LA）和甲基丙烯酸（MA）作为功能单体，乙二醇二甲基丙烯酸酯作为交联剂。同时，合成了非印迹聚合物（NIPs）。通过扫描电子显微镜、傅里叶变换红外反射、比表面积、孔径和释放动力学对 MIPs 和 NIPs 进行了表征。证明了它们对金黄色葡萄球菌和大肠杆菌的有效性以及对真皮成纤维细胞的细胞毒性。结果表明，MIPs 是介孔材料，孔径在 10 到 20nm 之间。发现使用 MA 作为单体的共沉淀 MIP 具有更高的吸附能力。释放动力学表明发生了非菲克扩散过程，并且使用 LA 的共沉淀 MIP 在 8 小时内表现出最高的释放速率（90.51mg/L）。最低抑菌浓度（MIC）对金黄色葡萄球菌在 0.031 至 0.016mg/L 之间，对大肠杆菌在 0.004 至 0.031mg/L 之间。在细胞培养物中未发现细胞毒性，并且细胞生长也得到了促进。本研究表明，MIPs 具有作为药物给药的有前途的特性，并可在临床实践中应用。
