Snabes M C, Kelch R P
Neuroendocrinology. 1979;29(1):34-41. doi: 10.1159/000122902.
To evaluate the dependence of pulsatile secretion of luteinizing hormone (LH) on gonadotropin-releasing hormone (GnRH), the acute effects of immunoneutralization of endogenous GnRH on plasma LH were compared in ovariectomized rats with phenobarbital treatment and hypophysectomy. Anti-GnRH produced a rapid dose-dependent decrease in plasma LH and LH pulse amplitude. Pulsatile secretion of LH was eliminated in 6 of 12 rats treated with the highest dose of anti-GnRH, but plasma LH was still 50% of control values 3 h after treatment. Frequency of LH pulses was unchanged in animals which had persistence of pulsatile secretion of LH. Phenobarbital eliminated pulsatile secretion of LH transiently. Hypophysectomized rats displayed a striking decrease in plasma LH which could be resolved into two exponential components with half-lives of 16 and 70 min. The initial half-life of plasma LH from untreated rats determined after LH pulses was also 16 min. These studies support the hypothesis that pulses of GnRH induce the pulsatile pattern of plasma LH and may be responsible for all LH secretion in ovariectomized rats.
为评估促黄体生成素（LH）的脉冲式分泌对促性腺激素释放激素（GnRH）的依赖性，在接受苯巴比妥治疗和垂体切除的去卵巢大鼠中，比较了内源性GnRH免疫中和对血浆LH的急性影响。抗GnRH使血浆LH和LH脉冲幅度迅速出现剂量依赖性下降。在接受最高剂量抗GnRH治疗的12只大鼠中，有6只的LH脉冲式分泌被消除，但治疗后3小时血浆LH仍为对照值的50%。LH脉冲分泌持续存在的动物中，LH脉冲频率未发生变化。苯巴比妥可短暂消除LH的脉冲式分泌。垂体切除的大鼠血浆LH显著下降，这可分解为半衰期分别为16分钟和70分钟的两个指数成分。在LH脉冲后测定的未处理大鼠血浆LH的初始半衰期也是16分钟。这些研究支持以下假设：GnRH脉冲诱导血浆LH的脉冲式模式，并且可能是去卵巢大鼠中所有LH分泌的原因。
