Yu J Y, Namiki H, Gorbman A
Neuroendocrinology. 1979;29(1):54-65. doi: 10.1159/000122905.
A study was made to compare the LH and FSH release patterns from isolated adult rat pituitaries in response to exposure to acidic extracts of rat hypothalamus or brain or to acidic extracts of GnRH-depleted hypothalamus, cerebral cortex, or cerebellum of adult (90-100 days old) or young (14 days old) rats of both sexes, using a continuous perifusion system. Hypothalami of adult rats contained 3-5 ng of radioimmunoreactive GnRH, while the corresponding cerebral cortex and cerebellum contained none, or extremely low levels of GnRH. Adult hypothalamic extract (HE) stimulated considerably greater LH and FSH release than was induced by cortical extract (CE) or cerebellar extract (CBE). Removal of assayable GnRH from HE by incubation with anti-GnRH serum reduced, but did not eliminate, release of both LH and FSH, suggesting that GnRH is the principal but not sole agent responsible for both LH and FSH secretions. Adult male HE released slightly more LH and FSH than did female HE due to its greater GnRH content. GnRH-depleted CE or CBE from both sexes induced LH and FSH releases qualitatively and quantitatively similar to those induced by CE, CBE or GnRH-depleted HE. Untreated extracts or GnRH-depleted extracts of all three brain regions from 14-day-old rats of both sexes induced similar LH and FSH releases as those of adult CE or CBE. Hypothalamus of young rats, which contained about 0.5 ng of immunoassayable GnRH did not release more LH and FSH than the corresponding cortex or cerebellum did. The data indicate that all brain regions so far studied, regardless of sex or age, contained presumably nonspecific substance(s), other than GnRH, capable of stimulating minor but significant LH and FSH releases. Their nature or role in physiological regulation of gonadotropin secretion is unknown, but must be considered, since current concepts, in which GnRH is the sole hypothalamic gonadotropin regulator, are not adequate.
采用连续灌流系统，对成年大鼠分离的垂体在暴露于成年（90 - 100日龄）或幼年（14日龄）雌雄大鼠下丘脑、脑、GnRH耗竭的下丘脑、大脑皮层或小脑的酸性提取物时促黄体生成素（LH）和促卵泡生成素（FSH）的释放模式进行了比较研究。成年大鼠下丘脑含有3 - 5 ng放射免疫活性GnRH，而相应的大脑皮层和小脑则不含或仅含极低水平的GnRH。成年下丘脑提取物（HE）刺激LH和FSH释放的程度比皮层提取物（CE）或小脑提取物（CBE）大得多。用抗GnRH血清孵育从HE中去除可检测的GnRH后，LH和FSH的释放量减少但并未消除，这表明GnRH是负责LH和FSH分泌的主要但非唯一因素。成年雄性HE由于其GnRH含量较高，释放的LH和FSH略多于雌性HE。来自两性的GnRH耗竭的CE或CBE诱导的LH和FSH释放，在质量和数量上与CE、CBE或GnRH耗竭的HE诱导的相似。来自14日龄两性大鼠的所有三个脑区的未处理提取物或GnRH耗竭提取物诱导的LH和FSH释放与成年CE或CBE诱导的相似。幼年大鼠下丘脑含有约0.5 ng可免疫检测的GnRH，其释放的LH和FSH并不比相应的皮层或小脑多。数据表明，迄今为止所研究的所有脑区，无论性别或年龄，除GnRH外，可能都含有非特异性物质，能够刺激少量但显著的LH和FSH释放。它们的性质或在促性腺激素分泌生理调节中的作用尚不清楚，但必须予以考虑，因为目前认为GnRH是下丘脑唯一促性腺激素调节因子的概念并不充分。
