Legay F, Henry S, Tappaz M
J Neurochem. 1987 Apr;48(4):1022-6. doi: 10.1111/j.1471-4159.1987.tb05620.x.
Immunoblots of the soluble proteins from a rat brain high-speed supernatant dissociated under reducing conditions showed two monomers (molecular weights, 59,000 and 62,000 +/- 2,000) immunolabeled by a glutamic acid decarboxylase (GAD) antiserum. In this extract, a GAD monoclonal antibody trapped the same two monomers, thus confirming that they are both constitutive subunits of GAD. Without treatment under reducing conditions, two additional bands were stained by immunoblotting. Their molecular weights were estimated to be 115,000 and 122,000 +/- 5,000. These results demonstrate the presence, in rat brain soluble extract, of two distinct forms of native GAD. They further support our previous hypothesis that each form is composed by the homodimeric association of each constitutive subunit through disulfide bridges.
在还原条件下解离的大鼠脑高速上清液中的可溶性蛋白质的免疫印迹显示，有两种单体（分子量分别为59,000和62,000±2,000）被谷氨酸脱羧酶（GAD）抗血清免疫标记。在该提取物中，一种GAD单克隆抗体捕获了相同的两种单体，从而证实它们都是GAD的组成亚基。在非还原条件下处理时，免疫印迹显示出另外两条带被染色。它们的分子量估计为115,000和122,000±5,000。这些结果证明在大鼠脑可溶性提取物中存在两种不同形式的天然GAD。它们进一步支持了我们之前的假设，即每种形式都是由每个组成亚基通过二硫键形成的同型二聚体缔合而成。
