新种，从铅锌矿尾矿中分离得到，以及将两个物种重新分类为新组合1和新组合2 。（注：这里原文中“comb. nov. and comb. nov.”未明确具体编号，所以翻译中保留原文表述形式并添加注释说明，实际应用中应根据具体编号准确翻译。）

sp. nov., isolated from lead-zinc mine tailing, and reclassification of two species as comb. nov. and comb. nov.

作者信息

Zhu Guo-Xing, Chen Xiu, Wu Ya-Jie, Wang Hai-Long, Lu Chun-Mei, Wang Xiao-Ming, Zhang Yue, Liu Zi-Chao, He Jiang-Bo, Tang Shu-Kun, Cao Yan-Ru

机构信息

College of Agriculture and Life Sciences & School of Medicine, Kunming University, Kunming, Yunnan, PR China.

Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, Yunnan, PR China.

出版信息

Int J Syst Evol Microbiol. 2024 Jan;74(1). doi: 10.1099/ijsem.0.006221.

DOI:10.1099/ijsem.0.006221

PMID:38197783

Abstract

A Gram-positive, acid-fast, aerobic, rapidly growing and non-motile strain was isolated from lead-zinc mine tailing sampled in Lanping, Yunnan province, Southwest China. 16S rRNA gene sequence analysis showed that the most closely related species of strain KC 300 was CGMCC 4.5724 (98.47 %). Additionally, phylogenomic and specific conserved signature indel analysis revealed that strain KC 300 should be a member of genus , and CECT 8779 and DSM 104744 should also members of genus . The genome size of strain KC 300 was 6.2 Mb with an DNA G+C content of 69.2 mol%. Chemotaxonomic characteristics of strain KC 300 were also consistent with the genus . The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values, as well as phenotypic, physiological and biochemical characteristics, support that strain KC 300 represents a new species within the genus , for which the name sp. nov. is proposed, with the type strain KC 300 (=CGMCC 1.19494=JCM 35915). In addition, we reclassified and as comb. nov. and comb. nov., respectively.

摘要

从中国西南部云南省兰坪采集的铅锌矿尾矿中分离出一株革兰氏阳性、抗酸、需氧、快速生长且不运动的菌株。16S rRNA基因序列分析表明，菌株KC 300与CGMCC 4.5724的亲缘关系最为密切（相似度为98.47%）。此外，系统基因组学和特定保守特征插入缺失分析表明，菌株KC 300应属于某属，CECT 8779和DSM 104744也应属于该属。菌株KC 300的基因组大小为6.2 Mb，DNA G+C含量为69.2 mol%。菌株KC 300的化学分类特征也与该属一致。平均核苷酸同一性、数字DNA-DNA杂交和平均氨基酸同一性值，以及表型、生理和生化特征均支持菌株KC 300代表该属内的一个新物种，为此提出新物种名称sp. nov.，模式菌株为KC 300（=CGMCC 1.19494=JCM 35915）。此外，我们分别将某菌株和某菌株重新分类为comb. nov.和comb. nov.。