两种新的快速生长分枝杆菌种，sp. nov. 和 sp. nov.。

sp. nov. and sp. nov., two new rapidly growing mycobacterial species.

机构信息

Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.

出版信息

Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005019.

DOI:10.1099/ijsem.0.005019

PMID:34878372

Abstract

Four bacterial strains (LJ126/S18 and Z-34/S20) recovered from faecal samples of Tibetan antelopes on the Qinghai-Tibet Plateau of China were analysed using a polyphasic approach. All four isolates were aerobic, short rod-shaped, non-motile, Gram-stain-positive, acid-fast and fast-growing. Phylogenetic analyses based upon 16S rRNA and whole-genome sequences showed that the two pair of strains formed two distinct branches within the evolutionary radiation of the genus . Strains LJ126/S18 and Z-34/S20 were most closely related to CCUG 37667, NCTC 10437, DSM 44605, JCM 15658, JCM 30395, JCM 6373 and JCM 13392, but readily distinguished from the known species by a combination of chemotaxonomic and phenotypic features and by low average nucleotide identity values (74.4-84.9 %). Consequently, the two strain pairs are considered to represent different novel species of for which the names sp. nov. and sp. nov. are proposed, with LJ126 (=CGMCC 1.1992=KCTC 49535) and Z-34 (=CGMCC 1.1993=DSM 106172) as the respective type strains.

摘要

从中国青藏高原藏羚羊粪便样本中分离得到的 4 株细菌（LJ126/S18 和 Z-34/S20）采用多相分析方法进行了分析。所有 4 株分离株均为需氧、短杆状、非运动性、革兰氏阳性、耐酸和快速生长。基于 16S rRNA 和全基因组序列的系统发育分析表明，这两对菌株在属内的进化辐射中形成了两个不同的分支。菌株 LJ126/S18 和 Z-34/S20 与 CCUG 37667、NCTC 10437、DSM 44605、JCM 15658、JCM 30395、JCM 6373 和 JCM 13392 最为密切相关，但通过化学分类和表型特征的组合以及低平均核苷酸同一性值（74.4-84.9%）与已知种容易区分。因此，这两对菌株被认为代表了的两个不同新种，分别命名为 sp. nov. 和 sp. nov.，LJ126（=CGMCC 1.1992=KCTC 49535）和 Z-34（=CGMCC 1.1993=DSM 106172）分别为相应的模式菌株。