Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai Japan.
Monoclon Antib Immunodiagn Immunother. 2024 Feb;43(1):24-31. doi: 10.1089/mab.2023.0016. Epub 2024 Jan 10.
CD39 is involved in adenosine metabolism by converting extracellular ATP to adenosine. As extracellular adenosine plays a critical role in the immune suppression of the tumor microenvironment, the inhibition of CD39 activity by monoclonal antibodies (mAbs) is one of the important strategies for tumor therapy. This study developed specific and sensitive mAbs for mouse CD39 (mCD39) using the Cell-Based Immunization and Screening method. The established anti-mCD39 mAb, CMab-1 (rat IgG, kappa), reacted with mCD39-overexpressed Chinese hamster ovary-K1 (CHO/mCD39) by flow cytometry. The kinetic analysis using flow cytometry indicated that the dissociation constant of CMab-1 for CHO/mCD39 was 7.3 × 10 M. Furthermore, CMab-1 detected the lysate of CHO/mCD39 by western blot analysis. These results indicated that CMab-1 is useful for the detection of mCD39 in many functional studies.
CD39 通过将细胞外 ATP 转化为腺苷参与腺苷代谢。由于细胞外腺苷在肿瘤微环境的免疫抑制中起着关键作用，因此单克隆抗体（mAbs）抑制 CD39 的活性是肿瘤治疗的重要策略之一。本研究使用基于细胞的免疫接种和筛选方法开发了用于小鼠 CD39（mCD39）的特异性和灵敏性 mAbs。建立的抗 mCD39 mAb，CMab-1（大鼠 IgG，kappa），通过流式细胞术与过表达 mCD39 的中国仓鼠卵巢-K1（CHO/mCD39）反应。使用流式细胞术进行的动力学分析表明，CMab-1 与 CHO/mCD39 的解离常数为 7.3×10 M。此外，CMab-1 通过 western blot 分析检测了 CHO/mCD39 的裂解物。这些结果表明，CMab-1 可用于许多功能研究中 mCD39 的检测。
