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Cby3/ciBAR1 复合物在精子发生过程中使环沿着精子鞭毛定位。

The Cby3/ciBAR1 complex positions the annulus along the sperm flagellum during spermiogenesis.

机构信息

Molecular and Cellular Biology Graduate Program, Stony Brook University, Stony Brook, NY, USA.

Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.

出版信息

J Cell Biol. 2024 Mar 4;223(3). doi: 10.1083/jcb.202307147. Epub 2024 Jan 10.

DOI:10.1083/jcb.202307147
PMID:38197861
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10783431/
Abstract

Proper compartmentalization of the sperm flagellum is essential for fertility. The annulus is a septin-based ring that demarcates the midpiece (MP) and the principal piece (PP). It is assembled at the flagellar base, migrates caudally, and halts upon arriving at the PP. However, the mechanisms governing annulus positioning remain unknown. We report that a Chibby3 (Cby3)/Cby1-interacting BAR domain-containing 1 (ciBAR1) complex is required for this process. Ablation of either gene in mice results in male fertility defects, caused by kinked sperm flagella with the annulus mispositioned in the PP. Cby3 and ciBAR1 interact and colocalize to the annulus near the curved membrane invagination at the flagellar pocket. In the absence of Cby3, periannular membranes appear to be deformed, allowing the annulus to migrate over the fibrous sheath into the PP. Collectively, our results suggest that the Cby3/ciBAR1 complex regulates local membrane properties to position the annulus at the MP/PP junction.

摘要

正确分隔精子鞭毛对于生育能力至关重要。环是一种基于 septin 的环，用于划定中段 (MP) 和主段 (PP)。它在鞭毛基部组装，向尾部迁移，并在到达 PP 时停止。然而，调节环定位的机制尚不清楚。我们报告说，Chibby3 (Cby3)/Cby1 相互作用的 BAR 结构域包含蛋白 1 (ciBAR1) 复合物是这个过程所必需的。在小鼠中敲除这两种基因都会导致精子鞭毛弯曲，环在 PP 中错位，从而导致雄性生育能力缺陷。Cby3 和 ciBAR1 相互作用并共定位到鞭毛口袋中弯曲膜内陷附近的环上。在没有 Cby3 的情况下，环周膜似乎变形，允许环迁移到纤维鞘中进入 PP。总之，我们的结果表明，Cby3/ciBAR1 复合物调节局部膜特性，将环定位在 MP/PP 交界处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/271f4cd7cddc/JCB_202307147_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/f623aafa38d3/JCB_202307147_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/3cd006d11430/JCB_202307147_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/87824e49658f/JCB_202307147_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/acd06a21857f/JCB_202307147_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/c5b49b72aca7/JCB_202307147_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/99f186379dbf/JCB_202307147_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/9fbc0a66a453/JCB_202307147_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/0c6b86e48499/JCB_202307147_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/8fd8a67da8bc/JCB_202307147_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/2a835ec9ba49/JCB_202307147_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/04c1e8fc1d5f/JCB_202307147_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/94e706f87417/JCB_202307147_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/271f4cd7cddc/JCB_202307147_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/f623aafa38d3/JCB_202307147_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/3cd006d11430/JCB_202307147_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/87824e49658f/JCB_202307147_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/acd06a21857f/JCB_202307147_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/c5b49b72aca7/JCB_202307147_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/99f186379dbf/JCB_202307147_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/9fbc0a66a453/JCB_202307147_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/0c6b86e48499/JCB_202307147_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/8fd8a67da8bc/JCB_202307147_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/2a835ec9ba49/JCB_202307147_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/04c1e8fc1d5f/JCB_202307147_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/94e706f87417/JCB_202307147_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b57f/10783431/271f4cd7cddc/JCB_202307147_Fig8.jpg

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