Star A M, Iannotti J P, Brighton C T, Armstrong P F
J Orthop Res. 1987;5(1):122-7. doi: 10.1002/jor.1100050115.
The cytosolic free calcium ion concentration for mammalian cell systems is believed to be maintained within a narrow range compatible with cellular homeostasis. Growth plate chondrocytes have been shown to accumulate large quantities of calcium within their mitochondria, but the cytosolic free calcium concentration has not been determined. This study measures the cytosolic free ionic calcium concentration in growth plate chondrocytes using two variations of the Quin II fluorescence technique. The results indicate that in isolated growth plate chondrocytes, the cytosolic free ionic calcium concentration is similar to other nonmineralizing mammalian cell types (106-137 mM).
对于哺乳动物细胞系统，胞质游离钙离子浓度据信维持在与细胞稳态相符的狭窄范围内。生长板软骨细胞已被证明会在其线粒体内积累大量钙，但胞质游离钙浓度尚未确定。本研究使用喹啉 II 荧光技术的两种变体来测量生长板软骨细胞中的胞质游离离子钙浓度。结果表明，在分离的生长板软骨细胞中，胞质游离离子钙浓度与其他非矿化哺乳动物细胞类型相似（106 - 137 mM）。
