State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Meishan Campus, Ningbo University, Ningbo, 315211, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Meishan Campus, Ningbo University, Ningbo, 315211, China.
Fish Shellfish Immunol. 2024 Feb;145:109364. doi: 10.1016/j.fsi.2024.109364. Epub 2024 Jan 8.
Micropterus salmoides rhabdovirus (MSRV) is one of the main pathogens of largemouth bass, leading to serious economic losses. The G protein, as the only envelope protein present on the surface of MSRV virion, contains immune-related antigenic determinants, thereby becoming the primary target for the design of MSRV vaccines. Here, we displayed the G protein on the surface of yeast cells (named EBY100/pYD1-G) and conducted a preliminary assessment of the protective efficacy of the recombinant yeast vaccine. Upon oral vaccination, a robust immune response was observed in systemic and mucosal tissue. Remarkably, following the MSRV challenge, the relative percent survival of EBY100/pYD1-G treated largemouth bass significantly increased to 66.7 %. In addition, oral administration inhibited viral replication and alleviated the pathological symptoms of MSRV-infected largemouth bass. These results suggest that EBY100/pYD1-G could be used as a potential oral vaccine against MSRV infection.
斑点叉尾鮰呼肠孤病毒（MSRV）是引起大口黑鲈严重经济损失的主要病原体之一。G 蛋白作为 MSRV 病毒粒子表面唯一的包膜蛋白，含有免疫相关的抗原决定簇，因此成为 MSRV 疫苗设计的主要目标。在这里，我们将 G 蛋白展示在酵母细胞表面（命名为 EBY100/pYD1-G），并对重组酵母疫苗的保护效力进行了初步评估。经口服免疫后，在系统和黏膜组织中观察到强烈的免疫反应。值得注意的是，在 MSRV 攻毒后，经 EBY100/pYD1-G 处理的大口黑鲈的相对存活率显著增加到 66.7%。此外，口服给药抑制了病毒复制，并缓解了 MSRV 感染大口黑鲈的病理症状。这些结果表明，EBY100/pYD1-G 可用作针对 MSRV 感染的潜在口服疫苗。
