The Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.
Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang, China.
Genomics. 2024 Mar;116(2):110784. doi: 10.1016/j.ygeno.2024.110784. Epub 2024 Jan 9.
Bacterial wilt (BW) caused by Ralstonia solanacearum is a globally prevalent bacterial soil-borne disease. In this study, transcriptome sequencing were subjected to roots after infection with the R. solanacearum in the resistant and susceptible tobacco variety. DEGs that responded to R. solanacearum infection in both resistant and susceptible tobacco contributed to pectinase and peroxidase development and were enriched in plant hormone signal transduction, signal transduction and MAPK signalling pathway KEGG terms. Core DEGs in the resistant tobacco response to R. solanacearum infection were enriched in cell wall, membrane, abscisic acid and ethylene terms. qRT-PCR indicated that Nitab4.5_0004899g0110, Nitab4.5_0004234g0080 and Nitab4.5_0001439g0050 contributed to the response to R. solanacearum infection in different resistant and susceptible tobacco. Silencing the p450 gene Nitab4.5_0001439g0050 reduced tobacco resistance to bacterial wilt. These results improve our understanding of the molecular mechanism of BW resistance in tobacco and solanaceous plants.
青枯病（BW）是由青枯雷尔氏菌引起的一种全球性的细菌性土传病害。在本研究中，对感病和抗病烟草品种感染青枯雷尔氏菌后的根系进行了转录组测序。响应感病和抗病烟草中青枯雷尔氏菌感染的差异表达基因（DEGs）有助于果胶酶和过氧化物酶的发展，并富集在植物激素信号转导、信号转导和 MAPK 信号通路 KEGG 术语中。抗病烟草对青枯雷尔氏菌感染的核心 DEGs 富集在细胞壁、膜、脱落酸和乙烯等术语中。qRT-PCR 表明，Nitab4.5_0004899g0110、Nitab4.5_0004234g0080 和 Nitab4.5_0001439g0050 有助于不同感病和抗病烟草对青枯雷尔氏菌感染的响应。沉默 p450 基因 Nitab4.5_0001439g0050 降低了烟草对青枯病的抗性。这些结果提高了我们对烟草和茄科植物 BW 抗性分子机制的理解。
