Histology and Embryology Department, Faculty of Medicine, Bilecik Seyh Edebali University, Bilecik, Turkey.
Department of Medical Services and Techniques, Yunus Emre Vocational School of Health Services, Anadolu University, Eskisehir, Turkey.
Biol Trace Elem Res. 2024 Nov;202(11):5000-5005. doi: 10.1007/s12011-023-04055-7. Epub 2024 Jan 11.
Osteoporosis and resulting fractures affect a significant group of people in the world. It has been shown in many studies that selenium has positive effects on bone metabolism. Based on this information, the aim of this study is to investigate whether bone differentiation will start in a shorter time by applying selenomethionine (SeMet) to hFOB cells.First, hFOB 1.19 cells were cultured. Safe doses of SeMet were determined by MTT and LDH tests. Ossification levels were determined by alizarin red staining and measurement of alkaline phosphatase enzyme levels. The results were analyzed with statistical tests.It was observed that SeMet increased cell viability at concentrations of 10, 25, 50, 100, and 200 µM in 24 h. At these concentrations, cell viability increased above the control, the viabilities were as follows: 109.4%, 104.9%, 104.3%, 103.15%, and 100.27%. High doses of SeMet significantly reduce cell viability. According to Alizarin red staining, SeMet increases the amount of calcium deposits in hFOB cells in a dose-dependent manner. In the experimental groups, the highest ALP enzyme was determined in the 7-day SeMet application. The most effective dose was measured as 15 µM.It was determined that SeMet, which is found as a trace element in living things in nature, increases the viability of hFOB cells, which are osteoblast cell precursors, and increases osteoblastic differentiation and osteoblastic activity in these cells. Our results are at a level that sheds light on an important problem in public health.
骨质疏松症及其导致的骨折影响着全球很大一部分人群。许多研究表明，硒对骨骼代谢有积极影响。基于这一信息，本研究旨在探讨向 hFOB 细胞施加硒代蛋氨酸（SeMet）是否能使骨分化更快开始。
首先，培养 hFOB 1.19 细胞。通过 MTT 和 LDH 试验确定 SeMet 的安全剂量。通过茜素红染色和碱性磷酸酶酶水平的测量来确定成骨水平。用统计检验分析结果。
结果表明，在 24 小时内，SeMet 在浓度为 10、25、50、100 和 200 μM 时增加细胞活力。在这些浓度下，细胞活力高于对照，活力分别为：109.4%、104.9%、104.3%、103.15%和 100.27%。高剂量的 SeMet 显著降低细胞活力。根据茜素红染色，SeMet 以剂量依赖的方式增加 hFOB 细胞中钙沉积物的量。在实验组中，在 SeMet 应用 7 天后测定最高的 ALP 酶。测定最有效剂量为 15 μM。
研究结果表明，在自然界中作为微量元素存在于生物体内的 SeMet 增加了成骨细胞前体 hFOB 细胞的活力，并增加了这些细胞中的成骨分化和成骨活性。我们的研究结果在一定程度上揭示了公共卫生领域的一个重要问题。
