Schilling-Hazlett Ashley, Raynor Edward J, Thompson Logan, Velez Juan, Place Sara, Stackhouse-Lawson Kim
CSU AgNext, Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Department of Animal Science and Industry, Kansas State University, Manhattan, KS 66502, USA.
Animals (Basel). 2023 Dec 19;14(1):9. doi: 10.3390/ani14010009.
The objective of this experiment was to demonstrate the effectiveness of a commercially available tannin product (Silvafeed ByPro, 70% tannic acid) as an enteric methane (CH) mitigation and preventative animal health strategy in Holstein heifers (BW = 219 ± 17 kg; 9 mo), reared under organic production system requirements. Twenty heifers were randomly assigned to one of four commercial tannin supplementation treatments as follows: 0% (0 g/hd/d; CON), 0.075% (5 g/hd/d; LOW), 0.15% (10 g/hd/d; MED), and 0.30% (~21 g/hd/d; HIG) of dry matter intake (DMI). Heifers received their treatment in individual animal feeding stanchions and were fed a basal total mixed ration (TMR) through four SmartFeed Pro intake measurement bunk systems (C-Lock Inc., Rapid City, SD, USA) for 45 d. An automatic head chamber system (AHCS; i.e., GreenFeed, C-Lock Inc., Rapid City, SD, USA) was used to continuously evaluate enteric CH production. No effect was observed among the treatments for CH emissions ( ≥ 0.55), animal performance ( ≥ 0.38), or oxidative stress biomarker concentration ( ≥ 0.55). Superoxide dismutase (SOD) and reduced glutathione (GSH) concentrations exhibited a linear response to increasing tannin dose ( = 0.003), indicating a potential tannin effect on the antioxidant status of dairy heifers. This observation may encourage future tannin research relating to animal health, which may be of particular interest to organic dairy systems. The results of this study suggest that tannin supplementation at 0%, 0.075%, 0.15%, and 0.30% of DMI, did not alter CH emissions, animal performance, or oxidative stress biomarker concentration in organic Holstein heifers when assessed under an on-farm research approach. Further, the results of this study affirm the challenges associated with on-farm research and the development of climate-smart strategies that are capable of mitigating climate impacts in less controlled environments under standard working conditions.
本实验的目的是证明一种市售单宁产品（Silvafeed ByPro，70% 单宁酸）作为一种减少肠道甲烷（CH）排放和预防动物健康问题的策略，在按照有机生产系统要求饲养的荷斯坦小母牛（体重 = 219 ± 17 千克；9 月龄）中的有效性。20 头小母牛被随机分配到以下四种商业单宁补充处理之一：干物质摄入量（DMI）的 0%（0 克/头/天；CON）、0.075%（约 5 克/头/天；LOW）、0.15%（约 10 克/头/天；MED）和 0.30%（约 21 克/头/天；HIG）。小母牛在个体动物饲养栏中接受处理，并通过四个 SmartFeed Pro 摄入量测量饲槽系统（C-Lock 公司，美国南达科他州拉皮德城）饲喂基础全混合日粮（TMR），持续 45 天。使用自动头部腔室系统（AHCS；即 GreenFeed，C-Lock 公司，美国南达科他州拉皮德城）连续评估肠道 CH 产量。在 CH 排放（≥ 0.55）、动物性能（≥ 0.38）或氧化应激生物标志物浓度（≥ 0.55）方面，各处理之间未观察到影响。超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）浓度对单宁剂量增加呈线性反应（P = 0.003），表明单宁对奶牛小母牛的抗氧化状态可能有影响。这一观察结果可能会鼓励未来开展与动物健康相关的单宁研究，这可能对有机奶牛系统特别有意义。本研究结果表明，在农场研究方法下评估时，DMI 的 0%、0.075%、0.15% 和 0.30% 的单宁补充量不会改变有机荷斯坦小母牛的 CH 排放、动物性能或氧化应激生物标志物浓度。此外，本研究结果证实了农场研究以及制定能够在标准工作条件下较少控制的环境中减轻气候影响的气候智能策略所面临的挑战。
