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基于ACQUITY UPLC I-Class Plus/VION IMS QTof对湖羊宰后成熟过程中肉的变化研究

Changes in Meat of Hu Sheep during Postmortem Aging Based on ACQUITY UPLC I-Class Plus/VION IMS QTof.

作者信息

Xu Jie, Wang Qiang, Wang Yimeng, Bao Menghuan, Sun Xiaomei, Li Yongjun

机构信息

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

出版信息

Foods. 2024 Jan 4;13(1):174. doi: 10.3390/foods13010174.

Abstract

Meat and meat products have a critical role in the human diet as important high-nutrient foods that are widely consumed worldwide. This study evaluated the effects of postmortem aging on Hu sheep's meat quality in the (LD) muscle during postmortem aging. The samples were stored at 4 ± 1 °C; the meat quality was measured at 6 h, 12 h, 24 h, 36 h, 48 h, 72 h, 96 h, 120 h, 144 h, and 168 h of postmortem aging. The results showed that, during the postmortem aging process, the pH of the muscles first decreased and then increased, and the shear force first increased and then decreased. The muscle fiber skeleton began to degrade, and the overall meat quality was improved to some extent. In addition, through ACQUITY UPLC I-Class Plus IMS Qtof identification of the muscle samples at different time points during the postmortem maturation process of the meat of Hu sheep, a total of 2168 metabolites were identified, and 470 metabolites were screened based on the VIP, P, and FC values, of which 79 were involved in KEGG pathways. In addition, pathways such as sphingolipid metabolism, glycerophospholipid metabolism, phenylalanine metabolism, and fatty acid elongation and degradation play an important role in the metabolic product changes in the meat of Hu sheep throughout the entire maturation process. These findings provide some insights into the changes in meat quality during the post-slaughter maturation process of lake lamb.

摘要

肉类和肉制品作为重要的高营养食品,在人类饮食中起着关键作用,在全球范围内被广泛食用。本研究评估了宰后成熟对湖羊背最长肌(LD)肉质的影响。样本储存在4±1℃;在宰后成熟6小时、12小时、24小时、36小时、48小时、72小时、96小时、120小时、144小时和168小时时测定肉质。结果表明,在宰后成熟过程中,肌肉的pH值先下降后上升,剪切力先上升后下降。肌肉纤维骨架开始降解,整体肉质有一定程度的改善。此外,通过ACQUITY UPLC I-Class Plus IMS Qtof对湖羊肉宰后成熟过程中不同时间点的肌肉样本进行鉴定,共鉴定出2168种代谢物,并根据VIP、P和FC值筛选出470种代谢物,其中79种参与KEGG通路。此外,鞘脂代谢、甘油磷脂代谢、苯丙氨酸代谢以及脂肪酸延长和降解等通路在湖羊肉整个成熟过程中的代谢产物变化中起重要作用。这些发现为湖羊宰后成熟过程中肉质变化提供了一些见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ed7/10778791/3304d3010777/foods-13-00174-g001.jpg

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