Léveillé M C, Roberts K D, Chevalier S, Chapdelaine A, Bleau G
J Reprod Fertil. 1987 Jan;79(1):173-83. doi: 10.1530/jrf.0.0790173.
Using an immunofluorescence technique on ovarian sections, zona-immunoreactive components were detected in the cytoplasm of the oocyte from the beginning of its growth, when it is surrounded by only a thin squamous follicular cell layer, up to the end of its growth. In parallel with oocyte growth, the staining intensity decreased in the ooplasm. No staining was observed in the cytoplasm of the granulosa cells during normal follicular development in adult cyclic females. However, staining of the granulosa cells was observed at some stages of follicular development in immature females. This staining was especially evident in the ovaries of immature females (22 or 26 days old) stimulated with PMSG. In addition, the staining of the granulosa cells was consistently observed in ovaries showing an abnormal histology. Increased staining of the zona at its outer and inner regions could be distinguished in normal follicles, but when staining occurred on the granulosa cells no such pattern was observed over the zona matrix. These studies indicate that the oocyte itself but not the granulosa cells elaborates the native immunogenic material of the zona pellucida. The administration of PMSG at particular stages of ovarian differentiation interferes with follicular development leading to an abnormal extracellular assembly of the zona and its degradation (phagocytosis) by the surrounding granulosa cells.
利用免疫荧光技术对卵巢切片进行检测，从卵母细胞开始生长直至生长结束，在其细胞质中均检测到透明带免疫反应性成分。在这个过程中，卵母细胞仅被一层薄薄的扁平卵泡细胞层所包围。随着卵母细胞的生长，卵质中的染色强度逐渐降低。在成年周期性雌性动物正常卵泡发育过程中，颗粒细胞的细胞质中未观察到染色现象。然而，在未成熟雌性动物卵泡发育的某些阶段，观察到颗粒细胞有染色。在用孕马血清促性腺激素（PMSG）刺激的未成熟雌性动物（22或26日龄）的卵巢中，这种染色尤为明显。此外，在组织学异常的卵巢中始终观察到颗粒细胞的染色。在正常卵泡中，可以区分出透明带外层和内层区域染色增强的情况，但当颗粒细胞出现染色时，在透明带基质上未观察到这种模式。这些研究表明，卵母细胞自身而非颗粒细胞产生透明带的天然免疫原性物质。在卵巢分化的特定阶段给予PMSG会干扰卵泡发育，导致透明带异常的细胞外组装及其被周围颗粒细胞降解（吞噬）。
