Pashirova Tatiana N, Nemtarev Andrey V, Buzyurova Daina N, Shaihutdinova Zukhra M, Dimukhametov Mudaris N, Babaev Vasily M, Voloshina Alexandra D, Mironov Vladimir F
Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov St., 420088 Kazan, Russia.
Alexander Butlerov Institute of Chemistry, Kazan (Volga Region) Federal University, 18 Kremlevskaya St., 420008 Kazan, Russia.
Nanomaterials (Basel). 2023 Dec 24;14(1):55. doi: 10.3390/nano14010055.
Currently, increasing the efficiency of glioblastoma treatment is still an unsolved problem. In this study, a combination of promising approaches was proposed: (i) an application of nanotechnology approach to create a new terpene-modified lipid system (7% /), using soybean L-α-phosphatidylcholine, N-carbonyl-methoxypolyethylene glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine for delivery of the chemotherapy drug, temozolomide (TMZ, 1 mg/mL); (ii) use of TMZ associated with natural compounds-terpenes (1% /) abietic acid and . resin (). Different concentrations and combinations of terpene-lipid systems were employed to treat human cancer cell lines T 98G (glioblastoma), M-Hela (carcinoma of the cervix) and human liver cell lines (Chang liver). The terpene-lipid systems appeared to be unilamellar and of spherical shape under transmission electron microscopy (TEM). The creation of a TMZ-loaded terpene-lipid nanosystem was about 100 nm in diameter with a negative surface charge found by dynamic light scattering. The 74% encapsulation efficiency allowed the release time of TMZ to be prolonged. The modification by terpenes of TMZ-loaded lipid nanoparticles improved by four times the cytotoxicity against human cancer T 98G cells and decreased the cytotoxicity against human normal liver cells. Terpene-modified delivery lipid systems are of potential interest as a combination therapy.
目前，提高胶质母细胞瘤的治疗效率仍然是一个未解决的问题。在本研究中，提出了一种有前景的方法组合：（i）应用纳米技术方法创建一种新的萜烯修饰脂质系统（7%/），使用大豆L-α-磷脂酰胆碱、N-羰基-甲氧基聚乙二醇-2000)-1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺来递送化疗药物替莫唑胺（TMZ，1mg/mL）；（ii）使用与天然化合物——萜烯（1%/）松香酸和.树脂（）相关的TMZ。采用不同浓度和组合的萜烯-脂质系统来处理人癌细胞系T98G（胶质母细胞瘤）、M-Hela（宫颈癌）和人肝细胞系（Chang肝）。在透射电子显微镜（TEM）下，萜烯-脂质系统似乎是单层球形的。通过动态光散射发现，负载TMZ的萜烯-脂质纳米系统的直径约为100nm，表面带负电荷。74%的包封效率使TMZ的释放时间得以延长。负载TMZ的脂质纳米颗粒经萜烯修饰后，对人癌T98G细胞的细胞毒性提高了四倍，而对人正常肝细胞的细胞毒性降低。萜烯修饰的递送脂质系统作为一种联合疗法具有潜在的应用价值。
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