Selective contrast-enhancement of the cochlear duct glycocalyx after OsO4/K4Ru(CN)6 post-fixation.

Fixation-dependent selective contrast-enhancement of the cochlear duct (or endolymphatic) glycocalyx was observed in the guinea pig inner ear after post-fixation with osmium tetroxide-potassium rutheniumcyanide. Contrast of the perilymphatic glycocalyx, however, was not significantly enhanced. The possible factors underlying this phenomenon are discussed.