Xu Nannan, Liu Hui, Qu Chunmei, Wen Sai, Zou Wenlu, Chang Caiyun, Wang Gang
Department of Infectious Disease, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
Institute of Bacterial Disease, Jinan Center for Disease Control and Prevention, Jinan 250021, China.
Infect Med (Beijing). 2023 Sep 17;2(4):334-337. doi: 10.1016/j.imj.2023.09.002. eCollection 2023 Dec.
China is not considered as an endemic area of , so there is no routine clinical way to diagnose this infection. This study aims to determine whether 2 febrile patients who had a tick bite in East China were indeed infected with The citrate synthase gene () was amplified with universal rickettsial primers by real-time fluorescent PCR from the patients' blood samples. Nested PCR was used to amplify the outer membrane protein A gene () for positive specimens. PCR products were further identified and analyzed through nucleic acid sequencing. Positive amplification of the and genes was found in both patients. The nucleotide sequences (303 bp) of the gene of the 2 patients had high homology (99%) with the Indian tick typhus strain in GenBank. A more than 4-fold increase in IgG against provided supportive evidence of SFG Rickettsia infection. And the rapid recovery after doxycycline treatment also supported a rickettsial cause for the disease. Physicians in East China should be aware of human infections with PCR-based diagnostic methods offer a rapid and precise way to diagnose rickettsiosis, improving patient identification and management.
中国不被视为[病原体名称]的流行地区，因此没有常规的临床方法来诊断这种感染。本研究旨在确定在中国东部被蜱叮咬的两名发热患者是否确实感染了[病原体名称]。通过实时荧光PCR，使用通用立克次氏体引物从患者血液样本中扩增柠檬酸合酶基因（[基因名称]）。对阳性标本使用巢式PCR扩增外膜蛋白A基因（[基因名称]）。PCR产物通过核酸测序进一步鉴定和分析。两名患者均检测到[基因名称]和[基因名称]的阳性扩增。两名患者的[基因名称]基因的核苷酸序列（303 bp）与GenBank中印度蜱传斑疹伤寒菌株具有高度同源性（99%）。抗[病原体名称]的IgG增加超过4倍为斑点热群立克次氏体感染提供了支持性证据。强力霉素治疗后迅速康复也支持了该疾病由立克次氏体引起。中国东部的医生应意识到人类感染[病原体名称]的情况。基于PCR的诊断方法为诊断立克次体病提供了快速准确的方法，有助于改善患者的识别和管理。
