Abdeen Ahmed, Elsabagh Rasha, Elbasuni Sawsan S, Said Alshaimaa M, Abdelkader Afaf, El-Far Ali H, Ibrahim Samah F, Mihaela Ostan, Fericean Liana, Abdelfattah Abdelfattah M, El-Hewaity Mohamed, Elbarbary Nady, Kadah Amgad Y, Ibrahim Samar S
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.
Front Pharmacol. 2023 Dec 27;14:1291965. doi: 10.3389/fphar.2023.1291965. eCollection 2023.
Aflatoxins (AFT) are ubiquitous environmental pollutants that are extremely dangerous for both human beings as well as animals. A safe, effective, and considerate strategy is therefore credited with controlling AFT intoxication. Therefore, our study aimed to evaluate the mitigating properties of (ChV) against AFT-induced nephrotoxicity and altered egg quality. Quails were randomized into Control group (receiving a normal diet); ChV group (1 g/kg diet); AFT group (receiving an AFT-containing diet); and the AFT-ChV group were given both treatments. AFT provoked kidney injury, exhibited by increased renal biochemical parameters and reduced protein levels. Malondialdehyde (MDA) levels dramatically increased as a consequence of AFT exposure, and glutathione (GSH) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities were also decreased. Substantial up-modulation of the mRNA expression of the inflammatory cytokines (TNF-α, IL-1β, and IL-6) was additionally reported. Furthermore, AFT residues were detected in the egg compromising its quality and nutritional value. Contrarily, ChV supplemented diet suppressed the AFT-prompted oxidative stress and inflammation, together with enhancing the nutritional value and quality of eggs and decreasing AFT residues. These beneficial impacts are proposed to be attributed to its antioxidant and nutritional ingredients. The molecular docking dynamics confirmed the inflammatory and apoptotic protein targets for ChV. Our findings recommend that adding ChV supplements to foods might guard against nephrotoxicity brought on by AFT exposure.
黄曲霉毒素（AFT）是普遍存在的环境污染物，对人类和动物都极具危险性。因此，一种安全、有效且周全的策略被认为可用于控制AFT中毒。所以，我们的研究旨在评估（ChV）对AFT诱导的肾毒性及鸡蛋品质改变的缓解特性。鹌鹑被随机分为对照组（给予正常饮食）；ChV组（1克/千克饮食）；AFT组（给予含AFT的饮食）；AFT - ChV组则同时接受两种处理。AFT引发肾损伤，表现为肾脏生化参数升高和蛋白质水平降低。由于暴露于AFT，丙二醛（MDA）水平显著升高，谷胱甘肽（GSH）水平、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性也降低。另外还报道了炎性细胞因子（TNF -α、IL -1β和IL -6）的mRNA表达大幅上调。此外，在鸡蛋中检测到AFT残留，损害了其品质和营养价值。相反，补充ChV的饮食可抑制AFT引发的氧化应激和炎症，同时提高鸡蛋的营养价值和品质，并减少AFT残留。这些有益影响被认为归因于其抗氧化和营养成分。分子对接动力学证实了ChV的炎性和凋亡蛋白靶点。我们的研究结果表明，在食物中添加ChV补充剂可能预防AFT暴露所致的肾毒性。
