人工釉质龋病损在去矿化和再矿化条件下钙共沉淀物渗透的效果。

Effect of calcium-coacervate infiltration of artificial enamel caries lesions in de- and remineralizing conditions.

机构信息

Department of Operative, Preventive and Pediatric Dentistry, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Aßmannshauser Str. 4-6, Berlin 14197, Germany.

出版信息

J Dent. 2024 Mar;142:104838. doi: 10.1016/j.jdent.2024.104838. Epub 2024 Jan 9.

DOI:10.1016/j.jdent.2024.104838

PMID:38211686

Abstract

OBJECTIVES

Calcium-coacervate emulsions (CC) might be considered as mineral precursors to foster remineralization of carious dental hard tissues. This study analyzed the instant effect of repeated infiltration of artificial caries lesions with a CC emulsion as well as the effects of subsequent exposure of CC-infiltrated lesions to demineralizing and remineralizing environments.

METHODS

Bovine enamel specimens were partly covered with varnish to leave three exposed windows. Artificial enamel caries lesions were created (pH 4.95, 17d). Baseline controls (BL) were obtained by preparing a thin section of each specimen. Specimens were allocated to five groups. In three groups lesions were etched with 37 % phosphoric acid gel, infiltrated with dipotassium hydrogen phosphate and subsequently with a calcium coacervate emulsion, prepared by mixing CaCl ⋅ 2HO with polyacrylic acid sodium salt (PAA-Na). Subsequently, the infiltration effect was either analyzed immediately (Inf.) or after exposition to either de- (Inf.+DS) or remineralizing solution (Inf.+RS) for 10 or 20 days, respectively. In two control groups specimens were exposed to either DS or RS, respectively without prior CC infiltration. Integrated mineral loss [ΔZ(vol%×µm)] was analyzed using transverse microradiography (TMR).

RESULTS

Infiltration of enamel caries lesions with coacervate solution resulted in only subtle immediate mineral gain even if repeated. When exposed to demineralizing conditions, infiltrated lesions showed significantly less mineral loss compared to untreated controls (p < 0.05; Kruskal Wallis) and exhibited characteristic mineral depositions within the lesion body.

CONCLUSIONS

While immediate mineral gain by infiltration was only modest, the CC-emulsion might be able to prevent demineralization in acidic conditions.

CLINICAL SIGNIFICANCE

Calcium coacervates might act protective against further demineralization when infiltrated into enamel caries lesions.

摘要

目的

钙共沉淀物乳液（CC）可被视为促进龋坏牙体硬组织再矿化的矿物质前体。本研究分析了重复渗透 CC 乳液对人工龋损的即时作用，以及随后将 CC 渗透的龋损暴露于脱矿和再矿化环境的影响。

方法

牛牙釉质标本部分用清漆覆盖，留下三个暴露窗口。人工牙釉质龋损（pH4.95，17d）。基线对照（BL）通过制备每个标本的薄片获得。标本被分配到五组。在三组中，用 37%磷酸凝胶蚀刻病变，用磷酸氢二钾渗透，然后用氯化钙和聚天冬氨酸钠盐（PAA-Na）混合制备的钙共沉淀物乳液渗透。随后，分别立即分析渗透效果（Inf.）或在暴露于脱矿（Inf.+DS）或再矿化溶液（Inf.+RS）10 或 20 天后分析渗透效果。在两个对照组中，分别在没有 CC 渗透的情况下将标本暴露于 DS 或 RS。使用横向显微射线照相术（TMR）分析整合矿化损失[ΔZ（体积%×μm）]。