Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fujian Medical University, Fuzhou, China.
Department of Molecular Pathology, Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou, China.
Int J Cancer. 2024 May 1;154(9):1639-1651. doi: 10.1002/ijc.34844. Epub 2024 Jan 11.
TRPS1 is aberrantly expressed in a variety of tumors, including breast, prostate, and gastric cancers, and is strongly associated with tumorigenesis or prognosis. However, the role of TRPS1 in high grade serous ovarian carcinoma (HGSC) is unknown. We investigated the relationship between TRPS1 expression and clinicopathology in HGSC patients. The tumor-related regulatory mechanisms of TRPS1 was explored through in vivo and vitro experiments. The results showed that TRPS1 was highly expressed in HGSC compared to normal tissues. It was also linked to the cell proliferation index Ki67 and poor prognosis. In vivo experiments showed that knockdown of TRPS1 could inhibit tumor growth. In vitro experiments, knockdown of TRPS1 inhibited the proliferation of ovarian cancer cells. TRPS1 exerted its regulatory role as a transcription factor, binding to the PSAT1 promoter and promoting the expression of PSAT1 gene. Meanwhile, PSAT1 was positively correlated with CCND1 expression. These results suggest that TRPS1 affects HGSC proliferation and cell cycle by regulating PSAT1 and thus CCND1 expression.
TRPS1 在多种肿瘤中异常表达，包括乳腺癌、前列腺癌和胃癌，与肿瘤发生或预后密切相关。然而，TRPS1 在高级别浆液性卵巢癌（HGSC）中的作用尚不清楚。我们研究了 TRPS1 表达与 HGSC 患者临床病理的关系。通过体内和体外实验探讨了 TRPS1 的肿瘤相关调控机制。结果表明，与正常组织相比，TRPS1 在 HGSC 中高表达。它还与细胞增殖指数 Ki67 和不良预后相关。体内实验表明，敲低 TRPS1 可以抑制肿瘤生长。体外实验表明，敲低 TRPS1 抑制卵巢癌细胞的增殖。TRPS1 作为转录因子发挥其调节作用，与 PSAT1 启动子结合，促进 PSAT1 基因的表达。同时，PSAT1 与 CCND1 表达呈正相关。这些结果表明，TRPS1 通过调节 PSAT1 和 CCND1 的表达来影响 HGSC 的增殖和细胞周期。
