骨髓间充质干细胞对Sprague-Dawley大鼠脑出血后高尔基体保护作用的研究
Study on the protective effect of OM-MSCs on Golgi apparatus after intracerebral hemorrhage in Sprague-Dawley rats.
作者信息
Liu Junjiang, Hu Zhiping, Huang Yan, Zhang Yidan, Peng Dezhen
机构信息
Department of Geriatrics, Hunan Provincial People's Hospital (First Affiliated Hospital of Hunan Normal University) No. 61 Jiefang West Road, Changsha 410011, Hunan, The People's Republic of China.
Department of Neurology, The Second Xiangya Hospital of Central South University Changsha 410011, Hunan, The People's Republic of China.
出版信息
Am J Stem Cells. 2023 Dec 15;12(5):124-137. eCollection 2023.
INTRODUCTION
The present study aimed to assess alterations in apoptosis rate, Golgi morphology and GOLPH3 expression following intracerebral hemorrhage (ICH) both before and after intervention with OM-MSCs. The objective was to investigate the impact of ICH on Golgi apparatus (GA) stress and to explore the potential protective effects of OM-MSCs on GA following ICH.
MATERIAL AND METHODS
A total of 54 Sprague-Dawley rats were allocated into three experimental groups: sham operation group, ICH group and OM-MSCs group. ICH models were established by collagenase method while OM-MSCs were cultured in vitro. In OM-MSCs intervention group, one million OM-MSCs were stereotactically injected into unilateral striatum of rats 48 hours after ICH modeling while other two groups received an equivalent volume of PBS. Brain tissues were collected at 1 day, 3 day and 7 day post intervention and subsequently assessed for cellular apoptosis, morphological change of GA and expression of GOLPH3. The obtained data were subjected to statistical analysis by SPSS 21.0.
RESULTS
- Apoptosis rate in the 1 d and 3 d ICH groups was significantly higher compared to sham operation group (P < 0.05), but significantly lower compared to OM-MSCs intervention group (P < 0.05). 2. While no noticeable morphological changes were observed in sham operation group, GA in ICH group exhibited a significant increase fragmentation. After OM-MSCs intervention, the fragmentation of GA decreased significantly. 3. On 3 d, expression of GOLPH3 in ICH group was significantly higher than that in sham operation group (P < 0.05) but significantly lower than that of OM-MSCs intervention group (P < 0.05).
CONCLUSIONS
The rate of apoptosis, fragmentation of GA, and expression of GOLPH3 exhibited significant increases following ICH in SD rats. Conversely, all of these factors demonstrated significant decreases subsequent to early intervention with OM-MSCs, thereby exerting neuroprotective effects.
引言
本研究旨在评估脑出血（ICH）前后经OM间充质干细胞干预后凋亡率、高尔基体形态及GOLPH3表达的变化。目的是研究ICH对高尔基体（GA）应激的影响，并探讨OM间充质干细胞对ICH后GA的潜在保护作用。
材料与方法
将54只Sprague-Dawley大鼠分为三个实验组：假手术组、ICH组和OM间充质干细胞组。采用胶原酶法建立ICH模型，同时体外培养OM间充质干细胞。在OM间充质干细胞干预组，ICH建模后48小时将100万个OM间充质干细胞立体定向注射到大鼠单侧纹状体，而其他两组注射等量的PBS。干预后1天、3天和7天收集脑组织，随后评估细胞凋亡、GA的形态变化及GOLPH3的表达。所得数据采用SPSS 21.0进行统计分析。
结果
- ICH组1天和3天的凋亡率显著高于假手术组（P < 0.05），但显著低于OM间充质干细胞干预组（P < 0.05）。2. 假手术组未观察到明显的形态变化，而ICH组的GA出现显著增加的碎片化。OM间充质干细胞干预后，GA的碎片化显著减少。3. 在3天时，ICH组GOLPH3的表达显著高于假手术组（P < 0.05），但显著低于OM间充质干细胞干预组（P < 0.05）。
结论
SD大鼠ICH后凋亡率、GA碎片化及GOLPH3表达均显著增加。相反，早期用OM间充质干细胞干预后，所有这些因素均显著降低，从而发挥神经保护作用。
Zotero 插件