State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China.
Anal Chem. 2024 Jan 30;96(4):1402-1409. doi: 10.1021/acs.analchem.3c03036. Epub 2024 Jan 12.
Hyperplexing approaches have been aimed to meet the demand for large-scale proteomic analyses. Currently, the analysis capacity has expanded to up to 54 samples within a single experiment by utilizing different isotopic and isobaric reagent combinations. In this report, we propose a super multiplexed approach to enable the analysis of up to 102 samples in a single experiment, by the combination of our recently developed TAG-TMTpro and TAG-IBT16 labeling. We systematically investigated the identification and quantification performance of the 102-plex approach using the mixtures of and HeLa peptides. Our results revealed that all labeling series demonstrated accurate and reliable quantification performance. The combination of TAG-IBT16 and TAG-TMTpro approaches expands the multiplexing capacity to 102 plexes, providing a more multiplexed quantification method for even larger-scale proteomic analysis. Data are available via ProteomeXchange with the identifier PXD042398.
超多重策略旨在满足大规模蛋白质组学分析的需求。目前，通过利用不同的同位素和等压试剂组合，单个实验的分析能力已扩展至多达 54 个样本。在本报告中，我们提出了一种超级多重化方法，通过结合我们最近开发的 TAG-TMTpro 和 TAG-IBT16 标记，可在单个实验中分析多达 102 个样本。我们使用 和 HeLa 肽的混合物系统地研究了 102 重策略的鉴定和定量性能。我们的结果表明，所有标记系列均表现出准确可靠的定量性能。TAG-IBT16 和 TAG-TMTpro 方法的结合将多重化能力扩展到 102 重，为更大规模的蛋白质组学分析提供了更具多重化的定量方法。数据可通过 ProteomeXchange 以标识符 PXD042398 获得。
