The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, China.
School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Talanta. 2024 Apr 1;270:125622. doi: 10.1016/j.talanta.2024.125622. Epub 2024 Jan 11.
Alkaline phosphatase (ALP) is a zinc-containing metalloprotein that shows very great significance in clinical diagnosis, which can catalyze the hydrolysis of phosphorylated species. ALP has the potential to serve as a valuable biomarker for detecting liver dysfunction and bone diseases. On the other hand, ALP is an efficient biocatalyst to amplify detection signals in the enzyme-linked assay. It has always been a major research focus to develop novel biosensors that can detect ALP activity with high selectivity and sensitivity. There have been numerous reports on the development of biosensors to determine ALP activity using a phosphorylated DNA probe. Among them, various beneficial strategies, such as λ exonuclease-mediated cleavage reaction, terminal deoxynucleotidyl transferase-triggered DNA polymerization, and Klenow fragment polymerase-catalyzed elongation, are employed to generate amplified and more intuitive signal. This review discusses and summarizes the development and advances of biosensors for ALP activity detection that use a well-designed phosphorylated DNA probe, aiming to provide some guidelines for the design of more sophisticated sensing strategies that exhibit improved sensitivity, selectivity, and adaptability in detecting ALP activity.
碱性磷酸酶（ALP）是一种含锌的金属蛋白酶，在临床诊断中具有非常重要的意义，它可以催化磷酸化物质的水解。ALP 有可能成为检测肝功能障碍和骨骼疾病的有价值的生物标志物。另一方面，ALP 是一种有效的生物催化剂，可以在酶联测定中放大检测信号。开发具有高选择性和灵敏度的新型生物传感器来检测 ALP 活性一直是一个主要的研究重点。已经有许多关于使用磷酸化 DNA 探针来确定 ALP 活性的生物传感器的开发的报道。其中，采用了 λ 外切酶介导的切割反应、末端脱氧核苷酸转移酶引发的 DNA 聚合以及 Klenow 片段聚合酶催化的延伸等各种有益的策略，以产生放大和更直观的信号。本文讨论并总结了使用精心设计的磷酸化 DNA 探针的用于检测 ALP 活性的生物传感器的发展和进展，旨在为设计更复杂的传感策略提供一些指导，这些策略在检测 ALP 活性时具有更高的灵敏度、选择性和适应性。
