从对甲氨蝶呤耐药的中国仓鼠卵巢细胞中分离扩增的二氢叶酸还原酶结构域。
Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells.
作者信息
Looney J E, Hamlin J L
出版信息
Mol Cell Biol. 1987 Feb;7(2):569-77. doi: 10.1128/mcb.7.2.569-577.1987.
Abstract
We isolated overlapping recombinant cosmids that represent the equivalent of two complete dihydrofolate reductase amplicon types from the methotrexate-resistant CHO cell line CHOC400. The type I amplicons are 260 kilobases long, are arranged in head-to-tail fashion, and represent 10 to 15% of the amplicons in the CHOC400 genome. The type II amplicons are 220 kilobases long, are arranged in head-to-head and tail-to-tail configurations, and constituted the majority of the remaining amplicons in CHOC400 cells. The type II amplicon sequences are represented entirely within the type I unit. These are the first complete amplicons to be cloned from a mammalian cell line.
摘要
我们从耐甲氨蝶呤的中国仓鼠卵巢（CHO）细胞系CHOC400中分离出重叠的重组黏粒，这些黏粒代表了两种完整的二氢叶酸还原酶扩增子类型。I型扩增子长260千碱基，以头对头的方式排列，占CHOC400基因组中扩增子的10%至15%。II型扩增子长220千碱基，以头对头和尾对尾的形式排列，构成了CHOC400细胞中其余扩增子的大部分。II型扩增子序列完全包含在I型单元内。这些是首次从哺乳动物细胞系中克隆出的完整扩增子。
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