特定离子对水稻谷蛋白淀粉样纤维聚集行为和结构变化的影响。
Specific ions effect on aggregation behaviors and structural changes of amyloid fibrils from rice glutelin.
机构信息
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
出版信息
Food Chem. 2024 May 30;441:138351. doi: 10.1016/j.foodchem.2023.138351. Epub 2024 Jan 5.
Metal ions have been considered as an important factor on fibrils assembly. Herein, a comprehensive analysis of specific ions effect on fibril formation and structural changes was investigated. The addition of ions (except Zn) accelerated the aggregation kinetics of rice glutelin fibrils (RGFs) from 0.93 to 1.28-2.19 h. In addition, the fibrillization rate followed the order of NH > Li > Na > K > Cu > Mg > Ca > Zn. The highest yield and length of fibrils were observed with Ca, probably due to the ionic bridging effect and hydrated capacity of Ca. However, Cu reduced the fibrils yield, which was attributable to the fact that Cu disrupted β-sheet structure and inhibited the transition of monomer to fibrils. The polymorphism of fibrils was observed with different salts, and the light metals presented a superior effect on fibrils formation than heavy metals. Overall, this work will provide a further information into how to tune the structure of RGFs using various ions.
金属离子被认为是影响纤维组装的一个重要因素。在此，我们综合分析了特定离子对纤维形成和结构变化的影响。除锌以外的离子的加入加速了大米谷蛋白纤维（RGF）的聚集动力学，从 0.93 小时缩短至 1.28-2.19 小时。此外，纤维形成的速率遵循 NH > Li > Na > K > Cu > Mg > Ca > Zn 的顺序。用 Ca 得到了最高的纤维产率和长度，这可能是由于 Ca 的离子桥接作用和水合能力。然而，Cu 降低了纤维的产率，这归因于 Cu 破坏了β-折叠结构并抑制了单体向纤维的转变。不同盐类表现出纤维的多晶型性，轻金属对纤维形成的影响优于重金属。总的来说，这项工作将进一步了解如何使用各种离子来调节 RGFs 的结构。
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