Yue Qiong, Tang Chenxin, Li Xiaofan, Lv Wei, Liu Hong, Yue Hanpeng, Chen Yongzhi
School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China; Technical Center of Sewage Treatment Industry in Gansu Province, Lanzhou, 730070, China.
Gansu Qilianshan Pharmaceutical Co., Ltd, China.
Chemosphere. 2024 Mar;351:141192. doi: 10.1016/j.chemosphere.2024.141192. Epub 2024 Jan 11.
The coexistence of antibiotics with sulfide and nitrate is common in sewage. Thus, this study explored the removal performance of nitrate and sulfide, and the response of extracellular polymer substances (EPS) and the microbial community to the sulfide autotrophic denitrification (SAD) process under oxytetracycline (OTC) stress. In Phase Ⅰ, the SAD system showed favouranle performance (nitrate removal rate ＞ 92.57%, sulfide removal rate ＞ 97.75%). However, in Phase Ⅳ, at OTC concentrations of 10, 15, and 20 mg/L, the NRE decreased to 76.13%, 40.71%, 11.37%, respectively, and the SRE decreased to 97.58%, 97.09%, 92.84%, respectively. At OTC concentrations of 0, 10, 15, and 20 mg/L, the EPS content were 1.62, 1.75, 2.03, and 1.42 mg/gVSS, respectively. The results showed that SAD performance gradually deteriorated under OTC stress. In particular, when the OTC concentration was 20 mg/L, the EPS content was lower than that of the control test, which could be attributed to the occurrence of microbial death. Finally, high-throughput sequencing results showed that OTC exposure led to gradual domination by heterotrophic denitrifying bacteria.
抗生素与硫化物和硝酸盐在污水中并存的情况很常见。因此，本研究探讨了在土霉素（OTC）胁迫下，硫化物自养反硝化（SAD）过程中硝酸盐和硫化物的去除性能，以及胞外聚合物（EPS）和微生物群落的响应。在第一阶段，SAD系统表现出良好的性能（硝酸盐去除率＞92.57%，硫化物去除率＞97.75%）。然而，在第四阶段，当OTC浓度为10、15和20mg/L时，硝酸盐去除率分别降至76.13%、40.71%、11.37%，硫化物去除率分别降至97.58%、97.09%、92.84%。在OTC浓度为0、10、15和20mg/L时，EPS含量分别为1.62、1.75、2.03和1.42mg/gVSS。结果表明，在OTC胁迫下，SAD性能逐渐恶化。特别是当OTC浓度为20mg/L时，EPS含量低于对照试验，这可能是由于微生物死亡所致。最后，高通量测序结果表明，OTC暴露导致异养反硝化细菌逐渐占主导地位。
