Department of Biotechnology, Mahatma Gandhi Central University, East Champaran, Motihari, Bihar, 845401, India.
Department of Zoology, Mizoram University, Aizawl, Mizoram, 796 004, India.
Mol Biol Rep. 2024 Jan 14;51(1):103. doi: 10.1007/s11033-023-09157-y.
Heat stress is known to adversely affect testicular activity and manifest the pathogenesis of spermatogenesis. Morin hydrate is a plant-derived compound, which contains a wide range of biological activities. Thus, it is hypothesized that morin hydrate might have an ameliorative effect on heat-induced testicular impairment. There has not been any research on the impact of morin hydrate on heat-induced testicular damage.
The experimental mice were divided into four groups, groups1 as the normal control group (CN), and the second which underwent heat stress (HS) by immersing the lower body for 15 min in a thermostatically controlled water bath kept at 43 °C (HS), and third and fourth heat-stressed followed by two different dosages of morin hydrate 10 mg/kg (HSM10) and 100 mg/kg (HSM100) for 14 days.
Morin hydrate treatment at 10 mg/kg improved, circulating testosterone levels (increases 3βHSD), and oxidative stress along with improvement in the testis and caput and corpus epididymis histoarchitecture, however, both doses of morin hydrate improved sperm parameters. Morin hydrate treatment significantly increases germ cell proliferation, (GCNA, BrdU staining), expression of Bcl2 and decreases expression of active caspase 3. Heat stress also decreased the expression of AR, ER- α, and ER-β, and Morin hydrate treatment increased the expression of these markers in the 10 mg/kg treatment group.
Morin hydrate ameliorates heat-induced testicular impairment modulating testosterone synthesis, germ cell proliferation, and oxidative stress. These effects could be manifested by regulating androgen and estrogen receptors. However, the two doses showed differential effects of some parameters, which requires further investigations.
热应激已知会对睾丸活动产生不利影响，并表现出生精发生的发病机制。桑色素是一种植物衍生的化合物，具有广泛的生物活性。因此，据推测，桑色素可能对热诱导的睾丸损伤具有改善作用。目前还没有关于桑色素对热诱导的睾丸损伤影响的研究。
实验小鼠分为四组，第 1 组为正常对照组（CN），第 2 组通过将下半身浸入恒温水浴中 15 分钟来进行热应激，水温保持在 43°C（HS），第 3 组和第 4 组进行热应激，然后分别用 10mg/kg（HSM10）和 100mg/kg（HSM100）的两种不同剂量的桑色素处理 14 天。
10mg/kg 的桑色素处理可改善循环睾酮水平（增加 3βHSD）和氧化应激，同时改善睾丸和附睾头和体的组织形态学，然而，两种剂量的桑色素均可改善精子参数。桑色素处理可显著增加生殖细胞增殖（GCNA、BrdU 染色），表达 Bcl2 并降低活性 caspase 3 的表达。热应激还降低了 AR、ER-α 和 ER-β 的表达，而桑色素处理增加了 10mg/kg 处理组中这些标志物的表达。
桑色素通过调节雄激素和雌激素受体，改善热诱导的睾丸损伤，调节睾酮合成、生殖细胞增殖和氧化应激。这些作用可能通过调节雄激素和雌激素受体来表现。然而，两种剂量对某些参数表现出不同的作用，这需要进一步研究。
