Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, PR China; Hubei Province Clinical Research Center for Precision Medicine for Critical Illness, 430030 Wuhan, PR China.
Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, PR China; Hubei Province Clinical Research Center for Precision Medicine for Critical Illness, 430030 Wuhan, PR China.
Carbohydr Polym. 2024 Mar 15;328:121745. doi: 10.1016/j.carbpol.2023.121745. Epub 2023 Dec 30.
Angelica sinensis polysaccharide (ASP) possesses diverse bioactivities; however, its metabolic fate following oral administration remains poorly understood. To intuitively determine its intestinal digestion behavior after oral administration, ASP was labeled with fluorescein, and it was found to accumulate and be degraded in the cecum and colon. Therefore, we investigated the in vitro enzymatic degradation behavior and identified the products. The results showed that ASP could be degraded into fragments with molecular weights similar to those of the fragments observed in vivo. Structural characterization revealed that ASP is a highly branched acid heteropolysaccharide with AG type II domains, and its backbone is predominantly composed of 1,3-Galp, →3,6)-Galp-(1→6)-Galp-(1→, 1,4-Manp, 1,4-Rhap, 1,3-Glcp, 1,2,3,4-Galp, 1,3,4,6-Galp, 1,3,4-GalAp and 1,4-GlcAp, with branches of Araf, Glcp and Galp. In addition, the high molecular weight enzymatic degradation products (ASP H) maintained a backbone structure almost identical to that of ASP, but exhibited only partial branch changes. Then, the results of ethanol-induced acute liver injury experiments revealed that ASP and ASP H reduced the expression of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and malondialdehyde (MDA) and increased the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) levels, thereby relieving ethanol-induced acute liver injury.
当归多糖（ASP）具有多种生物活性；然而，其口服后的代谢途径仍知之甚少。为了直观地确定其口服后的肠道消化行为，将荧光素标记在 ASP 上，结果发现它在盲肠和结肠中积累并降解。因此，我们研究了其体外酶解行为并鉴定了产物。结果表明，ASP 可降解为分子量与体内观察到的片段相似的片段。结构表征表明，ASP 是一种高度分支的酸性杂多糖，具有 AG 型 II 结构域，其主链主要由 1,3-Galp、→3,6)-Galp-(1→6)-Galp-(1→、1,4-Manp、1,4-Rhap、1,3-Glcp、1,2,3,4-Galp、1,3,4,6-Galp、1,3,4-GalAp 和 1,4-GlcAp 组成，支链由 Araf、Glcp 和 Galp 组成。此外，高分子量酶解产物（ASP H）保持与 ASP 几乎相同的主链结构，但仅表现出部分支链变化。然后，乙醇诱导急性肝损伤实验结果表明，ASP 和 ASP H 降低了天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）和丙二醛（MDA）的表达，增加了超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的水平，从而缓解了乙醇诱导的急性肝损伤。
