Rai Mayank, Chucha Diezehlouno, Deepika Dake, Lap Bharati, Magudeeswari P, Padmavathi G, Singh Nagendra, Tyagi Wricha
School of Crop Improvement, College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University (Imphal), Umiam, India.
ICAR- Indian Institute of Rice Research, Hyderabad, Telangana India.
Physiol Mol Biol Plants. 2023 Dec;29(12):1931-1943. doi: 10.1007/s12298-023-01387-5. Epub 2023 Nov 21.
Water deficit is a crucial factor causing huge loss to rice productivity. The present study aimed to develop a multiple stress tolerant genotype by pyramiding drought tolerant yield QTLs qDTY and qDTY into high yielding rice variety Samba Mahsuri Sub-1(SMS) through marker assisted pyramiding. To achieve this six introgression lines of SMS carrying qDTY (SAB) were crossed with DRR-50, an Essentially Derived Variety of SMS carrying qDTY. The SAB lines are taller than SMS due to tight linkage between qDTY and wild type SD-1. Therefore, F generation of crosses were screened for recombinants between and qDTY. Phenotyping of 1530 F plants representing three F populations from 35 F hybrids, identified 305 dwarf plants. Three dwarf F plants along with three others carrying qDTY and qDTY. were forwarded to F generation. From the six F (SABD) lines fourteen pyramided progenies were selected and forward to F generation. The six SABD F lines SABD-7, SABD-8, SABD-9, SABD-76, SABD-79 and SABD-80 along with parents were evaluated under moisture stress (MS) for various physiological parameters. Chlorophyll and relative water content were more, while canopy temperature and malonaldehyde (MDA) content were lesser in SABD lines compared to parents indicating tolerance under MS. Variance due to genotypes was highly significant for all the yield related traits except test weight. Based on seed morphology, agronomic characters and physiological parameters six superior lines SABD-9-3, SABD-9-2, SABD-9-6, SABD-9-7, SABD-76-2 and SABD-76-6 performing better under MS were identified, which could be released after multi-location evaluation.
The online version contains supplementary material available at 10.1007/s12298-023-01387-5.
水分亏缺是导致水稻产量大幅损失的关键因素。本研究旨在通过标记辅助聚合，将耐旱产量QTLs qDTY和qDTY聚合到高产水稻品种桑巴马苏里Sub-1（SMS）中，培育出一种多胁迫耐受基因型。为实现这一目标，将携带qDTY的6个SMS导入系（SAB）与DRR-50杂交，DRR-50是携带qDTY的SMS基本衍生品种。由于qDTY与野生型SD-1紧密连锁，SAB系比SMS更高。因此，对杂交的F1代进行qDTY和之间重组体的筛选。对代表35个F1杂种的3个F2群体的1530株F2植株进行表型分析，鉴定出305株矮化植株。3株矮化F2植株以及另外3株携带qDTY和qDTY的植株被推进到F3代。从6个F3（SABD）系中选出14个聚合后代并推进到F4代。对6个SABD F4系SABD-7、SABD-8、SABD-9、SABD-76、SABD-79和SABD-80及其亲本在水分胁迫（MS）下的各种生理参数进行了评估。与亲本相比，SABD系的叶绿素和相对含水量更高，而冠层温度和丙二醛（MDA）含量更低，表明在水分胁迫下具有耐受性。除容重外，所有产量相关性状的基因型方差均极显著。根据种子形态、农艺性状和生理参数，鉴定出6个在水分胁迫下表现较好的优良品系SABD-9-3、SABD-9-2、SABD-9-6、SABD-9-7、SABD-76-2和SABD-76-6，经多点评估后可发布。
在线版本包含可在10.1007/s12298-023-01387-5获取的补充材料。
