Malta Luccas Gabriel Ferreira, Koerich Leonardo Barbosa, D'Ávila Pessoa Grasielle Caldas, Araujo Ricardo N, Sant'Anna Mauricio Roberto Viana, Pereira Marcos H, Gontijo Nelder Figueiredo
Laboratório de Fisiologia de Insetos Hematófagos, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Pampulha, Belo Horizonte, Minas Gerais, Brazil.
Laboratório de Fisiologia de Insetos Hematófagos, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Pampulha, Belo Horizonte, Minas Gerais, Brazil.
Comp Biochem Physiol A Mol Integr Physiol. 2024 Apr;290:111584. doi: 10.1016/j.cbpa.2024.111584. Epub 2024 Jan 14.
Clogmia albipunctata (Williston, 1893) is a non-hematophagous insect belonging to the order Diptera, suborder Nematocera (Lower Diptera) and family Psychodidae. In the present work, we investigated how C. albipunctata control their midgut pH under different physiological conditions, comparing their midgut physiology with some nematoceran hematophagous species. The C. albipunctata midgut pH was measured after ingestion of sugar, protein and under the effect of the alkalinizing hormone released in the hemolymph of the hematophagous sand fly Lutzomyia longipalpis obtained just after a blood meal. The midgut pH of unfed or sugar-fed C. albipunctata is 5.5-6, and its midgut underwent alkalinization after protein ingestion or under treatment with hemolymph collected from blood fed L. longipalpis. These results suggested that in nematocerans, mechanisms for pH control seem shared between hematophagous and non-hematophagous species. This kind of pH control is convenient for successful blood digestion. The independent evolution of many hematophagous groups from the Lower Diptera suggests that characteristics involved in midgut pH control were already present in non-hematophagous species and represent a readiness for adaptation to this feeding mode.
白点库蠓（Clogmia albipunctata，威利斯顿，1893年）是一种非吸血性昆虫，属于双翅目、长角亚目（低等双翅目）和蛾蠓科。在本研究中，我们研究了白点库蠓在不同生理条件下如何控制中肠pH值，并将其与一些长角亚目吸血性昆虫的中肠生理进行了比较。在摄入糖、蛋白质后，以及在吸食血液后的吸血沙蝇——长须罗蛉（Lutzomyia longipalpis）血淋巴中释放的碱化激素的作用下，测量了白点库蠓的中肠pH值。未进食或摄入糖的白点库蠓的中肠pH值为5.5 - 6，在摄入蛋白质后或用从吸食血液的长须罗蛉收集的血淋巴处理后，其中肠会发生碱化。这些结果表明，在长角亚目中，pH值控制机制似乎在吸血性和非吸血性物种之间是共享的。这种pH值控制有利于血液的成功消化。许多吸血类群从低等双翅目独立进化而来，这表明中肠pH值控制所涉及的特征在非吸血性物种中已经存在，并且代表了对这种取食方式的适应准备。
