Yin Fei, Zhou Yang, Xie Dongli, Liang Yunxia, Luo Xiaogang
College of Textile and Clothing Engineering, Soochow University, 199 Ren-Ai Road, Suzhou, 215123, China.
School of Textile Science and Engineering/National Engineering Laboratory for Advanced Yarn and Clean Production, Wuhan Textile University, Wuhan, 430200, China.
Environ Res. 2024 Apr 15;247:118106. doi: 10.1016/j.envres.2024.118106. Epub 2024 Jan 13.
Exposure to large-size particulate air pollution (PM2.5 or PM10) has been reported to increase risks of aging-related diseases and human death, indicating the potential pro-aging effects of airborne nanomaterials with ultra-fine particle size (which have been widely applied in various fields). However, this hypothesis remains inconclusive. Here, a meta-analysis of 99 published literatures collected from electronic databases (PubMed, EMBASE and Cochrane Library; from inception to June 2023) was performed to confirm the effects of nanomaterial exposure on aging-related indicators and molecular mechanisms in model animal C. elegans. The pooled analysis by Stata software showed that compared with the control, nanomaterial exposure significantly shortened the mean lifespan [standardized mean difference (SMD) = -2.30], reduced the survival rate (SMD = -4.57) and increased the death risk (hazard ratio = 1.36) accompanied by upregulation of ced-3, ced-4 and cep-1, while downregulation of ctl-2, ape-1, aak-2 and pmk-1. Furthermore, multi-transcriptome data associated with nanomaterial exposure were retrieved from Gene Expression Omnibus (GSE32521, GSE41486, GSE24847, GSE59470, GSE70509, GSE14932, GSE93187, GSE114881, and GSE122728) and bioinformatics analyses showed that pseudogene prg-2, mRNAs of abu, car-1, gipc-1, gsp-3, kat-1, pod-2, acdh-8, hsp-60 and egrh-2 were downregulated, while R04A9.7 was upregulated after exposure to at least two types of nanomaterials. Resveratrol (abu, hsp-60, pod-2, egrh-2, acdh-8, gsp-3, car-1, kat-1, gipc-1), naringenin (kat-1, egrh-2), coumestrol (egrh-2) or swainsonine/niacin/ferulic acid (R04A9.7) exerted therapeutic effects by reversing the expression levels of target genes. In conclusion, our study demonstrates the necessity to use phytomedicines that target hub genes to delay aging for populations with nanomaterial exposure.
据报道，暴露于大尺寸颗粒物空气污染（PM2.5或PM10）会增加与衰老相关疾病的风险和人类死亡风险，这表明具有超细粒径的空气中纳米材料（已广泛应用于各个领域）具有潜在的促衰老作用。然而，这一假设仍无定论。在此，我们对从电子数据库（PubMed、EMBASE和Cochrane图书馆；从创刊到2023年6月）收集的99篇已发表文献进行了荟萃分析，以确认纳米材料暴露对模式动物秀丽隐杆线虫中与衰老相关指标和分子机制的影响。通过Stata软件进行的汇总分析表明，与对照组相比，纳米材料暴露显著缩短了平均寿命[标准化平均差（SMD）=-2.30]，降低了存活率（SMD=-4.57），并增加了死亡风险（风险比=1.36），同时ced-3、ced-4和cep-1上调，而ctl-2、ape-1、aak-2和pmk-1下调。此外，从基因表达综合数据库（GSE32521、GSE41486、GSE24847、GSE59470、GSE70509、GSE14932、GSE93187、GSE114881和GSE122728）中检索了与纳米材料暴露相关的多转录组数据，生物信息学分析表明，在暴露于至少两种类型的纳米材料后，假基因prg-2、abu、car-1、gipc-1、gsp-3、kat-1、pod-2、acdh-8、hsp-60和egrh-2的mRNA下调, 而R04A9.7上调。白藜芦醇（abu、hsp-60、pod-2、egrh-2、acdh-8、gsp-3、car-1、kat-1、gipc-1）、柚皮素（kat-1、egrh-2）、香豆雌酚（egrh-2）或苦马豆素/烟酸/阿魏酸（R04A9.7）通过逆转靶基因的表达水平发挥治疗作用。总之，我们的研究表明，对于暴露于纳米材料的人群，有必要使用靶向关键基因的植物药来延缓衰老。
