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[凝集试验(AT)、补体结合试验(CFT)和抗球蛋白试验(AGT)在猪布鲁氏菌病诊断中的评估。III. 基础研究]

[Evaluation of the agglutination test (AT), complement fixation test (CFT) and the antiglobulin test (AGT) in the diagnosis of swine brucellosis. III. Basic studies].

作者信息

Karpiński T M, Zórawski C, Skwarek P

出版信息

Pol Arch Weter. 1986;24(3):301-13.

PMID:3822855
Abstract

In the examinations of the swine sera obtained from swines immunized s.c. with adjuvant Br.abortus S19 vaccine or Br.suis 1417 vaccine, it was found that agglutinins were present after 3 weeks, and C.F. antibodies or incomplete agglutinins normally after injections. Probably, in the first period of Brucella infection negative results of C.F.T. or AGT or both will be obtained. In the swine sera from Brucella free herds, agglutinins reacting with the Brucellognost antigen were present. The performance of mercaptoethanol test or C.F.T. lead in most cases to suitable diagnosis. In our conditions we have not obtained results which permit to classify AGT as a supplement test in serodiagnosis of swine brucellosis.

摘要

在对经皮下注射流产布鲁氏菌S19佐剂疫苗或猪布鲁氏菌1417疫苗免疫的猪所采集血清进行检测时发现,3周后出现凝集素,通常注射后出现补体结合(C.F.)抗体或不完全凝集素。在布鲁氏菌感染的第一阶段,可能会得到补体结合试验(C.F.T.)或凝集试验(AGT)或两者的阴性结果。在来自无布鲁氏菌猪群的猪血清中,存在与布鲁氏菌诊断抗原发生反应的凝集素。巯基乙醇试验或补体结合试验的结果在大多数情况下有助于做出合适的诊断。在我们的条件下,尚未获得能将凝集试验归类为猪布鲁氏菌病血清诊断补充试验的结果。

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