Yu Jie, Li Shengnan, Li Mizhuan, Zhang Yujie, Tong Dayan, Xu Yuzhu, Wang Chengxing, Xu Jie
School of Public Health, Zunyi Medical University, No. 6 Xuefu Road, Xinpu New District, Zunyi City, Guizhou Province 5643006, P. R. of China.
Toxicol Res (Camb). 2024 Jan 13;13(1):tfae003. doi: 10.1093/toxres/tfae003. eCollection 2024 Feb.
This study aimed to investigate the effects of exposure to nonylphenol (NP) on anxiety/depression-like behaviors in rats and alleviation of those effects via green tea and zinc selenium (Zn-Se) tea interventions.
Totally, 40 male specific-pathogen free (SPF) Sprague-Dawley (SD) male rats were randomly divided into four groups (n = 10 rats per group): control group (5 ml/kg corn oil), NP group (40 mg/kg NP), NP + GT group (40 mg/kg NP + 1 g/kg/day green tea), and NP + Zn-Se tea group (40 mg/kg NP + 1 g/kg/day ZST). All dose-based groups received oral gavage of either corn oil or drugs over a 6-month period: NP at a dosage of 40 mg/kg/day was administered to rats for the initial 3 months, followed by a combination of NP with green tea and NP with Zn-Se tea for the subsequent 3 months.
Tea intervention resulted in weight loss in rats. The hippocampal tissue NP level in the tea group was slightly lower than that in the NP group. Following tea intervention, compared with the NP group, the residence time in the light-dark box test was shortened = 0.048, < 0.001), and the number of entries into the closed arm in the elevated plus maze test in the tea-treated group was significantly reduced. In addition, the immobility time in the central square in the open field test decreased. The sucrose preference index score in the sucrose preference test increased, and the immobility time in the forced swimming test was reduced ( = 0.049, < 0.001). The effects of Zn-S e tea were superior to green tea. The damage to the hippocampal tissues in the group treated with tea was less than that in the NP group. The cellular arrangement was tighter with degeneration, deepstaining, and pyknotic nerve cells were visible. The nuclei in the NP group were atrophied, and the cells were sparsely arranged. Compared with the control group, serum brain-derived neurotrophic factor (BDNF) level was lower in the NP group. The serum corticosterone level in the NP group was elevated. Compared with the NP group, serum corticosterone level was reduced in the NP + Zn-Se tea group.
Chronic NP exposure induced anxiety/depression-like behaviors in rats. Green tea effectively reduced the damage to the hippocampus and prefrontal cortex induced by NP. The effects of Zn-Se tea were slightly more noticeable than those of conventional green tea.
本研究旨在探讨暴露于壬基酚（NP）对大鼠焦虑/抑郁样行为的影响，以及通过绿茶和锌硒（Zn-Se）茶干预减轻这些影响。
总共40只雄性无特定病原体（SPF）斯普拉格-道利（SD）大鼠随机分为四组（每组n = 10只大鼠）：对照组（5 ml/kg玉米油）、NP组（40 mg/kg NP）、NP + GT组（40 mg/kg NP + 1 g/kg/天绿茶）和NP + Zn-Se茶组（40 mg/kg NP + 1 g/kg/天Zn-Se茶）。所有基于剂量的组在6个月内接受玉米油或药物的口服灌胃：在最初3个月给大鼠施用剂量为40 mg/kg/天的NP，随后3个月将NP与绿茶以及NP与Zn-Se茶联合使用。
茶干预导致大鼠体重减轻。茶组海马组织中的NP水平略低于NP组。茶干预后，与NP组相比，明暗箱试验中的停留时间缩短（P = 0.048，P < 0.001），茶处理组高架十字迷宫试验中进入封闭臂的次数显著减少。此外，旷场试验中中央广场的不动时间减少。蔗糖偏好试验中的蔗糖偏好指数得分增加，强迫游泳试验中的不动时间减少（P = 0.049，P < 0.001）。Zn-Se茶的效果优于绿茶。茶处理组海马组织的损伤小于NP组。细胞排列更紧密，可见细胞变性、深染和固缩的神经细胞。NP组细胞核萎缩，细胞排列稀疏。与对照组相比，NP组血清脑源性神经营养因子（BDNF）水平较低。NP组血清皮质酮水平升高。与NP组相比，NP + Zn-Se茶组血清皮质酮水平降低。
慢性NP暴露诱导大鼠出现焦虑/抑郁样行为。绿茶有效减轻了NP对海马和前额叶皮质的损伤。Zn-Se茶的效果比传统绿茶稍明显。
1）慢性NP暴露诱导大鼠出现焦虑/抑郁样行为。2）Zn-Se茶减轻了NP对海马和前额叶皮质的损伤。3）NP诱导的抑郁伴随着BDNF、CORT和神经病理学的变化。
