Department of Biological Sciences, College of Natural and Applied Sciences, Crescent University, Abeokuta, Ogun State, Nigeria.
RIMT University, Mandi Gobindgarh, India.
J Basic Microbiol. 2024 Mar;64(3):e2300589. doi: 10.1002/jobm.202300589. Epub 2024 Jan 17.
Current research was performed to look for the performance of Bacillus cereus PY3 for metal detoxification. Strain PY3 was recognized as B. cereus using 16 S rRNA. Higher rate of removal of Zn and Cr (VI) by PY3 was obtained between pH 6-8 and 100-500 µg/mL in 24 h. Highest removal of Cr by strain PY3 was achieved at acidic, neutral, and alkaline atmosphere, 100-300 µg Cr /mL and 25-35°C. Supernatant of PY3 detoxified Cr into Cr then cell pellet (debris) adsorbed them. The mechanism of metal removal was due to the release of cytolic extracts. Release of antioxidants and bio-film played a protective role against cell damage. Metals increased antioxidants and bio-film formation. SEM images showed the smooth external structure of PY3 when cells were exposed to metals thus confirming the role of cells for detoxification. Results Above facts conclude that PY3 can remove metallic pollution in polluted soil.
目前的研究旨在寻找蜡状芽孢杆菌 PY3 用于金属解毒的性能。通过 16S rRNA 鉴定，菌株 PY3 被认定为蜡状芽孢杆菌。PY3 在 pH 6-8 和 100-500μg/mL 之间，24 小时内对 Zn 和 Cr(VI)的去除率较高。在酸性、中性和碱性环境中，在 100-300μg Cr/mL 和 25-35°C 时，菌株 PY3 对 Cr 的去除率最高。PY3 的上清液将 Cr 解毒为 Cr，然后细胞沉淀（碎片）吸附它们。金属去除的机制是由于细胞质提取物的释放。抗氧化剂和生物膜的释放对细胞损伤起到了保护作用。金属增加了抗氧化剂和生物膜的形成。SEM 图像显示了 PY3 细胞暴露于金属时的光滑外部结构，从而证实了细胞在解毒中的作用。上述结果表明，PY3 可以去除污染土壤中的金属污染。
