State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People's Republic of China.
College of Engineering and Applied Sciences, Nanjing University, Jiangsu, 210093, People's Republic of China.
Mikrochim Acta. 2024 Jan 18;191(2):104. doi: 10.1007/s00604-023-06126-x.
A lateral flow assay (LFA) strip based on dual 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB)-encoded satellite FeO@Au (Mag@Au) SERS tags with nanogap is reported for ultrasensitive and simultaneous diagnosis of two SARS-CoV-2 functional proteins. Composed of FeO core, satellite gold shell with nanogaps, and double-layer DTNB, the Mag@Au nanoparticles with an average size of 238 nm were designed as multifunctional tags to efficiently enrich the target SARS-CoV-2 protein from complex samples, significantly enhancing the SERS signal of the LFA strip and provide quantitative SERS detection of analyte on test lines. The developed dual DTNB-encoded satellite Mag@Au-based LFA allowed simultaneous quantification of spike (S) protein and nucleocapsid (NP) protein with detection limits of 23 pg mL and 2 pg mL, respectively, lower than commercial ELISA kits and reported SERS-LFA detection system-based Au NPs and FeO@3 nm Au MNPs. This magnetic SERS-LFA also showed high performance of multi-variant strain detection and further distinguished clinical samples of Omicron variant infection, demonstrating the potential of in situ detection of respiratory virus diseases.
基于双 5,5'-二硫代双(2-硝基苯甲酸)(DTNB)编码卫星 FeO@Au(Mag@Au)具有纳米间隙的表面增强拉曼散射(SERS)标签的侧向流动测定法(LFA)条带，用于超灵敏和同时诊断两种 SARS-CoV-2 功能蛋白。由 FeO 核、具有纳米间隙的卫星金壳和双层 DTNB 组成，平均尺寸为 238nm 的 Mag@Au 纳米颗粒被设计为多功能标签，可从复杂样品中有效富集靶 SARS-CoV-2 蛋白，显著增强 LFA 条带的 SERS 信号，并提供分析物在测试线上的定量 SERS 检测。所开发的双 DTNB 编码卫星 Mag@Au 基 LFA 允许同时定量检测刺突(S)蛋白和核衣壳(NP)蛋白，检测限分别为 23pg mL 和 2pg mL，低于商业 ELISA 试剂盒和报道的基于 Au NPs 和 FeO@3nm Au MNPs 的 SERS-LFA 检测系统。这种磁性 SERS-LFA 还表现出多变体菌株检测的高性能，并进一步区分了奥密克戎变体感染的临床样本，展示了用于呼吸道病毒疾病原位检测的潜力。
