Department of Oral Diagnostic Sciences, Faculty of Health Sciences, Institute of Dentistry, University of Eastern Finland, Kuopio Campus, PO Box 1627, 70211, Kuopio, Finland.
Oral, Private Clinic, Lahti, Finland.
Clin Oral Investig. 2024 Jan 18;28(1):103. doi: 10.1007/s00784-023-05389-1.
The present preliminary study aimed to investigate the salivary metabolic profile in patients with asymptomatic oral lichen planus (OLP) using nuclear magnetic resonance (NMR) spectroscopy.
Stimulated whole mouth saliva (SWMS) samples were collected from 15 reticular OLP female patients and 15 from age- and sex-matched controls (HCs). A total of 23 metabolites were identified and quantified. Mann-Whitney's U test was used to compare the determined concentration salivary metabolite concentrations between OLP patients and the healthy controls.
The concentration of acetate, methylamine, and pyruvate was elevated, whereas the concentration of tyrosine was decreased in the saliva of OLP patients compared with HCs. To identify a combination of metabolites, multivariate discrimination function analysis (DFA) was conducted. DFA analysis have shown that the most powerful discrimination between the groups was achieved when methylamine and tyrosine were considered as combined biomarkers.
Salivary tyrosine was of particular interest and a promising finding for the screening of OLP and its progression. Further longitudinal studies are required to establish it as a reliable salivary biomarker in OLP.
The salivary metabolic profiling can describe the pathologic characteristics of OLP on non-invasive saliva samples and NMR analysis. Salivary metabolites provide details to considered early detectors and to impact oral health of OLP patients.
本初步研究旨在使用核磁共振（NMR）光谱法研究无症状口腔扁平苔藓（OLP）患者的唾液代谢谱。
收集了 15 名网状 OLP 女性患者和 15 名年龄和性别匹配的健康对照者（HCs）的全口刺激唾液（SWMS）样本。共鉴定和定量了 23 种代谢物。使用曼-惠特尼 U 检验比较 OLP 患者和健康对照者唾液代谢物浓度。
与 HCs 相比，OLP 患者唾液中的乙酸盐、甲胺和丙酮酸浓度升高，而酪氨酸浓度降低。为了确定一组代谢物，进行了多元判别函数分析（DFA）。DFA 分析表明，当将甲胺和酪氨酸视为组合生物标志物时，两组之间的区分最为有效。
唾液酪氨酸特别有趣，是筛查 OLP 及其进展的有前途的发现。需要进一步的纵向研究来确定其作为 OLP 可靠的唾液生物标志物。
唾液代谢组学分析可以在非侵入性唾液样本和 NMR 分析中描述 OLP 的病理特征。唾液代谢物提供了早期检测的详细信息，并影响 OLP 患者的口腔健康。
