Meng Xiaolin, Wu Ying, Tang Wangruiqi, Zhou Lei, Liu Wei, Liu Chengmei, Prakash Sangeeta, Zhang Yanjun, Zhong Junzhen
State Key Laboratory of Food Science and Resources, Nanchang University, No 235, Nanjing East Road, Nanchang, Jiangxi 330047, China.
School of Agriculture and Food Sustainability, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
Food Chem. 2024 Jun 1;442:138414. doi: 10.1016/j.foodchem.2024.138414. Epub 2024 Jan 14.
Based on the findings of our previous studies, a comprehensive comparative investigation of the quality and formation mechanism of gels obtained from protein self-assemblies induced by different methods is necessary. Self-assembled heat-induced gels had higher gel mechanical strength, and hydrophobic interactions played a greater role. Whether or not heat treatment was used to induce gel formation may play a more important role than the effect of divalent cations on gel formation. Hydrogen bonds played an important role in all gels formed using different gelation methods. Furthermore, Self-assembled cold-induced gels were considered to can load bioactive substances with different hydrophilicity properties due to the high water-holding capacity and the smooth, dense microstructure. Therefore, β-lactoglobulin fibrous and worm-like self-assembled cold-induced gels as a delivery material for hydrophilic bioactive substances (epigallocatechin gallate, vitamin B) and amphiphilic bioactive substance (naringenin), with good encapsulation efficiency (91.92 %, 97.08 %, 96.72 %, 96.52 %, 98.94 %, 97.41 %, respectively) and slow-release performance.
基于我们之前的研究结果，有必要对不同方法诱导蛋白质自组装得到的凝胶的质量和形成机制进行全面的比较研究。热诱导自组装凝胶具有更高的凝胶机械强度，疏水相互作用发挥了更大的作用。与二价阳离子对凝胶形成的影响相比，是否使用热处理来诱导凝胶形成可能发挥更重要的作用。氢键在所有使用不同凝胶化方法形成的凝胶中都起着重要作用。此外，由于高持水能力和平滑、致密的微观结构，冷诱导自组装凝胶被认为能够负载具有不同亲水性的生物活性物质。因此，β-乳球蛋白纤维状和蠕虫状自组装冷诱导凝胶作为亲水性生物活性物质（表没食子儿茶素没食子酸酯、维生素B）和两亲性生物活性物质（柚皮素）的递送材料，具有良好的包封效率（分别为91.92%、97.08%、96.72%、96.52%、98.94%、97.41%）和缓释性能。
