L-瓜氨酸具有抗肥胖和抗糖尿病作用，其作用具有性别依赖性。

Anti-obesity and anti-diabetic effects of L-citrulline are sex-dependent.

机构信息

Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Department of Molecular, Cellular and Biomedical Sciences, Sophie Davis School of Biomedical Education, City University of New York School of Medicine, NY, USA.

出版信息

Life Sci. 2024 Feb 15;339:122432. doi: 10.1016/j.lfs.2024.122432. Epub 2024 Jan 17.

DOI:10.1016/j.lfs.2024.122432

PMID:38237764

Abstract

AIMS

Anti-diabetic and anti-obesity effects of L-citrulline (Cit) have been reported in male rats. This study determined sex differences in response to Cit in Wistar rats.

MAIN METHODS

Type 2 diabetes (T2D) was induced using a high-fat diet followed by low-dose of streptozotocin (30 mg/kg) injection. Male and female Wistar rats were divided into 4 groups (n = 6/group): Control, control+Cit, T2D, and T2D + Cit. Cit (4 g/L in drinking water) was administered for 8 weeks. Obesity indices were recorded, serum fasting glucose and lipid profile were measured, and glucose and pyruvate tolerance tests were performed during the Cit intervention. White (WAT) and brown (BAT) adipose tissues were weighted, and the adiposity index was calculated at the end of the study.

KEY FINDINGS

Cit was more effective in decreasing fasting glucose (18 % vs. 11 %, P = 0.0100), triglyceride (20 % vs. 14 %, P = 0.0173), and total cholesterol (16 % vs. 11 %, P = 0.0200) as well as decreasing gluconeogenesis and improving glucose tolerance, in females compared to male rats with T2D. Following Cit administration, decreases in WAT weight (16 % vs. 14 % for gonadal, 21 % vs. 16 % for inguinal, and 18 % vs. 13 % for retroperitoneal weight, all P < 0.0001) and increases in BAT weight (58 % vs. 19 %, for interscapular and 10 % vs. 7 % for axillary, all P < 0.0001) were higher in females than male rats with T2D. The decrease in adiposity index was also higher (11 % vs. 9 %, P = 0.0007) in females.

SIGNIFICANCE

The anti-obesity and anti-diabetic effects of Cit in rats are sex-dependent, with Cit being more effective in female than male rats.

摘要

目的

已报道 L-瓜氨酸（Cit）具有抗糖尿病和抗肥胖作用。本研究旨在确定雄性大鼠中 Cit 反应的性别差异。

方法

使用高脂肪饮食加低剂量链脲佐菌素（30mg/kg）注射诱导 2 型糖尿病（T2D）。雄性和雌性 Wistar 大鼠分为 4 组（每组 n=6）：对照组、对照组+Cit、T2D 组和 T2D+Cit 组。Cit（饮用水中 4g/L）给药 8 周。记录肥胖指数，测量血清空腹血糖和血脂谱，并在 Cit 干预期间进行葡萄糖和丙酮酸耐量试验。测量白色（WAT）和棕色（BAT）脂肪组织的重量，并在研究结束时计算肥胖指数。

主要发现

与雄性 T2D 大鼠相比，Cit 更有效地降低空腹血糖（18%对 11%，P=0.0100）、甘油三酯（20%对 14%，P=0.0173）和总胆固醇（16%对 11%，P=0.0200），并降低糖异生和改善葡萄糖耐量。给予 Cit 后，T2D 雌性大鼠的 WAT 重量减少（性腺 16%对 14%，腹股沟 21%对 16%，腹膜后 18%对 13%，均 P<0.0001）和 BAT 重量增加（肩胛间 58%对 19%，腋窝 10%对 7%，均 P<0.0001）高于 T2D 雄性大鼠。肥胖指数的降低也更高（11%对 9%，P=0.0007）。